XB-CLONE-4353350
Clone Name: IMAGE:7026133
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Gene:
rps8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783668
[+]
taattaatcccccccccccacccccccgctctttccagccagcgcctgagaatgggtatctcaagggacaactggcacaagcgccgcaagactggtggca
aaaggaagccttatcacaaaaagagaaagtatgaacttggccgtcctgctgcaaacaccaagattggtcctcgccgtatccacactgtgagagtccgcgg
tggtaacaaaaagtatcgtgctctgaggttggatgctggcaacttttcatggggttcagagtgttgcacacgtaaaactaggatcattgatgtggtgtac
aatgcgtcaaacaatgagctggttcgaaccaaaacccttgtgaagaactgcattgtcctggttgacagcactccttacagacagtggtttgaggcccact
atgctctcccactaggacccaagaagggagctaaactcacccctgaagaggaagaaatcctaaacaagaaaacgatcttatgaaaacaccggaagaaatt
tggatggagaggaaaaaagacggcaaaaataaagcccactttttttgaaaaaacagtttccaggcaaggaaaaactggctttgcattgccataatcccct
ccaaaaacggggaaaaagtgttgtgtaaaaaccttgatgggttatggtacttgggaaggggaaaaggaattttggaattttccaaccctgtaaaaaaaaa
ttctaaggggctcaaaaaacggggggaaaataataaccttggttaatcaccacactttagtccagtcccaagatgaattggtttgctttgataaccccgt
caagttactagtaatgatggatgaggggtgtctctcctaaagtttctcttaaatagagggtgcccgccacatcccccaactggtagggaagaattttatg
agcgcgctgggagcacacaaa
3' EST: CF783669
[+]ttttttttttttttttgttttaataacatttatttgatgataagtgtggtaacatgtttatttcccctttttggccttgatctttctcaggtagaactcc
aactccttcccttccagtacataaccatcagctctaccacactgtcccggtctggaggctatgcatgcaagcagtttgccttgctggaactgttcttcca
aaagtgggcttatttttgctgtctttttcctctcctcatatttcttctgtgttttcttagatcgtttcttgtttaggatttcttcctcttcaggggtgag
tttagctcccttcttgcgtcctagtgggagagcatagtgggcctcaaaccactgtctgtaaggagtgctgtcaaccaggacaatgcagttcttcacaagg
gttttggttcgaaccagctcattgtttgacgcattgtacaccacatcaatgatcctagttttacgtgtgcaacactctgaaccccatgaaaagttgccag
catccaacctcagagcacgatactttttgttaccaccgcggactctcacagtgtggatacggcgaggaccaatcttggtgtttgcagcaggacggccaag
ttcatactttctctttttgtgataaggcttccttttttgcaccagtccttgcgggcgcttggtgccaatttgtccccttggagataccccatttctcaag
gcgccttggctgtgaaaaaagcggggggggggggggaggggggagggaaatttgtccaatccccggtggataaaagaggcacagagataattaaagatgg
tatgggaagactccactaagagatgactcgtggaggagcatagagcaatggacttgatgacgagtattgatatg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: