XB-CLONE-4353345
Clone Name: IMAGE:7026131
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Gene:
get1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077696
[+]
CCCCCCCGGATCGCTATTGTTGCAGCCGATGTGGGCGCTGCTGGGAAACATGGCGGAGAGCTGTGCGGCGTGGCTGTTAGTGCTGAGCGCCGTGTTTCTG
TGCAATCTCTGTAAAATCCTGCTTCCTTCTTTCTCCTCGCTTGTCTCCAGGCTACTACAGGAAGATGCGGAGCAGGAGTATGAGATGAGGTCTGATATCC
AGACCATGAGGCAAGAACTCTCTAATATCAGCATGATGGACGAGTTTGCCAAATACGCCAGATTGGAACGCAAAATCAACAAGATGACCGACAAACTTAA
AACACATGTAAAAAGCAGGACGGCACAACTATCAAAAATCAAGTGGGTCATCAGCATCGTCTTCTACGTACTACAGGCAGCCCTGATGGTGTCCCTCATA
TGGAAATATTACTCGGAGCCGGTCACCGTACTGCCCAGCAAATGGATCGCTCCGCTGGAGAGACTCGTGGCCTTTCCCACTGGCATTGCAGGTGGCGTCG
GAATTACTTGTTGGCTTGTGGTGTGCAACAAAGTGGTGGCCATCATGCTGTACCCTATCAGCTAAGAAGAATGGGTTCCATCAGTAGCGTTATCATAGTC
CCATCTGTAGGCATGTGTTTCTGTGCGAGTCATGTATTATCTGTGTGTTTATTATCTGTATAGTATTTATTCACATGTATGTTGCCTTATTTGCACTGTT
CTTTTATTAAACAGTGTGAAGTGCAAAAAAAAAAAAAAAA
5' EST: CF783671
[+]taattaatccccccccccccacccccccggatcgctattgttgcagccgatgtgggcgctgctgggaaacatggcggagagctgtgcggcgtggctgtta
gtgctgagcgccgtgtttctgtgcaatctctgtaaaatcctgcttccttctttctcctcgcttgtctccaggctactacaggaagatgcggagcaggagt
atgagatgaggtctgatatccagaccatgaggcaagaactctctaatatcagcatgatggacgagtttgccaaatacgccagattggaacgcaaaatcaa
caagatgaccgacaaacttaaaacacatgtaaaaagcaggacggcacaactatcaaaaatcaagtgggtcatcagcatcgtcttctacgtactacaggca
gccctgatggtgtccctcatatggaaatattactcggagccggtcaccgtactgcccagcaaatggatcgctccgctggagagactcgtggcctttccca
ctggcattgcaggtggcgtcggaattacttgttggcttgtggtgtgcaacaaagtggtggccatcatgctgtaccctatcagctaagaagaaaggggttc
catcagtagcgttatcatagttcccatctggtaggcatgttgtttctcgtgccgagacaagggaattatcctgtgggggtttaattaaccctgggataag
aatttttattcccccatggttatggttgcccctttattttggcccctgggttccttttttatttataaaccgggtgggtgaaaggtgggccaaataaaat
aaataaaaaaaaaagaggggatcccctctcttgggttttcttaagagagggggggccggcccacacaccgggcggaggggtggagaaacattttggtggg
cncccgtgtgngnccc
3' EST: CF783672
[+]tttttttttttttttgcacttcacactgtttaataaaagaacagtgcaaataaggcaacatacatgtgaataaatactatacagataataaacacacaga
taatacatgactcgcacagaaacacatgcctacagatgggactatgataacgctactgatggaacccattcttcttagctgatagggtacagcatgatgg
ccaccactttgttgcacaccacaagccaacaagtaattccgacgccacctgcaatgccagtgggaaaggccacgagtctctccagcggagcgatccattt
tgctgggcagtacngtgaccggctccgagtaatatttccatatgaaggacaacatccagggctgcctgtaatacgtaaaagaaccatgctgatgaaccac
ctgattttttgataagtgtgggcggcctgcctttttacaaggtgggtttaaagtttggccgggcaacctggttgaatttgcggttccaaactgggggtat
tttggcaaaaatcggtccattcaaggcgaaaattagaggaattccttggccccaggggctggggaatcaaaaaacccatttcataaatcccggggccccc
aaattttctgttaaaaacccccggagaacaagccccgggggaaaaaaaggggaaccccgggtttttttccaaaatttggtgcagaaaaaaacggggggct
ctagaacttaaaaagcccccccccgcccaaaaatttttctcggcattgggttttcccacaacaacgcgccccacaattttgggtgtggaaaaaaaaaaaa
agaattctccgggggggggnggnngnngnngnntan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: