XB-CLONE-4353330
Clone Name: IMAGE:7026126
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Gene:
prrc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783682 [+]
taattaatccccccccccccccccagtcagcaatagcagagcggacatattccatgccgggctataccattggaactgctaggggagggagtgattggca
tccatagctggtggctgactgtacctttgggcgtgtgttgggaatatcggatacttgctgaagaaacattagatactcttcactggctgcacttgtttcg
ggccccttttgcagaaaatgatggaagagagtggtattgaaacaacacctcctagcacacctcctcccagcactgctggaacatcagtagccgcagccac
aacagccatcgccacccctattccaccagttttgtctagccctctggcggcaccagcattctccccactgccatccttcgcacagccaagtttttctacc
ccagttccttcctcagttgctccaccaagatcatcagtccctttcacatatgcatctgcccttcctgtcactggggtgcactctcctcctgtcaacacct
ctgtacctgctgcttttagcagcccattgccagcattctcttccccatcatctttccctcctcctcccctgaacaccctccaggccccgtttttttcaac
acacccctgggttccccctgaaggaggaatcccctatgttttcccacttatcaattcacctaaaggggtctggtggggcgaaaccccctcaaaaccccct
ctgaatgcccaaaaaaattcttgaaccccctcggtttcaaatttttttcccaaaacccggtgccccctttttaaaaacccctcctctttttacacggggg
ggaggggggaatttttccccatttaacactttccccccggagaa
3' EST: CF783681 [+]tttttttttttttttcctcataaaaaggtttatttaaatctgatccaggtcactggtccatagcagtcaatgtgttagcaaaacgtcttcacttgggtat
aaaaagtgtactaaaataagtctgaacatttgtaagcatagaggagttagagacagcaatgtaatctagtttttaaggcaaagaacattacgttcacctt
tcacaagctaatcctgtcactgcacgtcactcttgtcttttacacttcattacttaggcttacatacactaatatagcctgtagtaactgtaggttatga
atgctaaaaacatggccattttataccaaagcatagtctgccaataaaaaatgaatacatgaatagaaaatcatttaaataatttactccattacaaaga
attgctatttcgaagggacactactaaaaatcccccaactccattcacagttatctgtattcagtgtcagatatcatgatggggcaggaaagaatttgca
tcatttgaattaagtggtgcaacatccattttgctcaattctaggatttactttagcacattgtttgtaaaaatgtgcctagacaaaagaattctacaaa
cagatctgcaccctatctgatttcaccatcgagtagcagttccagtagtgcaaatagggtatagcccaaagatcatgtgcatatgttgcacaaatgcaat
ccctaagggcttaaatggcaatattcgtctgtgtcacgtgataatgcaataacaatgcataattatggggaaatgtaatccatacttttgaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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