XB-CLONE-4353292
Clone Name: IMAGE:7026112
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Gene:
snrpf
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077006
[+]
TTTTTTTTCTCCTGTCGCTTCCGGGCCTTGGCAGCCATTTCCCGCTTTGCTCCAGCGGTGTGTTCTACGGGACGCTCCGGGGAGGAATTTTTTTTATTGA
TCTGCAGTTTGTTGTGAGGCTGCGGTCGCACCATGAGTTTACCACTGAATCCAAAACCCTTCCTTAATGGCCTCACTGGTAAGCCAGTTATGGTAAAGCT
GAAATGGGGGATGGAGTACAAAGGCTACTTGGTATCAGTGGATGGATACATGAACATGCAGCTTGCAAACACAGAAGAATATATTGATGGTGCATTGTCT
GGACATCTTGGAGAAGTTTTGATAAGATGCAACAATGTGTTATATATACGTGGGGTAGAAGAGGAGGAGGAAGATGGAGAGATGAGAGAGTAATTGGAAT
TATTTGGAAATCCCTTTGTATAGTTTCATGAAGAAAAGGATAACTTCATTTATTATGAAGGCATTTTTTTTACAGCATTTTTCTTTTTTTTATAGTTGTT
TACACTGCATTCAGTGTTTGTTCTTTATTGTGTTAGAACAATAAAAGATAATTGCATTGCAAAAAAAAAAAAAAAA
5' EST: CF783707
[+]taattaatcccccccccccccttttttttctcctgtcgcttccgggccttggcagccatttcccgctttgctccagcggtgtgttctacgggacgctccg
gggaggaattttttttattgatctgcagtttgttgtgaggctgcggtcgcaccatgagtttaccactgaatccaaaacccttccttaatggcctcactgg
taagccagttatggtaaagctgaaatgggggatggagtacaaaggctacttggtatcagtggatggatacatgaacatgcagcttgcaaacacagaagaa
tatattgatggtgcattgtctggacatcttggagaagttttgataagatgcaacaatgtgttatatatacgtggggtagaagaggaggaggaagatggag
agatgagagagtaattggaattatttggaaatccctttgtatagtttcatgaagaaaaggataacttcatttattatgaaggcattttttttacagcatt
tttcttttttttatagttgtttacactgcattcagtgtttgttctttattgtgttagaacaataaaagataattgcattgcaaaaaaaaaaaaaaaag
3' EST: CF783706
[+]tttttttttttttttgcaatgcaattatcttttattgttctaacacaataaagaacaaacactgaatgcagtgtaaacaactataaaaaaaagaaaaatg
ctgtaaaaaaaatgccttcataataaatgaagttatccttttcttcatgaaactatacaaagggatttccaaataattccaattactctctcatctctcc
atcttcctcctcctcttctaccccacgtatatataacacattgttgcatcttatcaaaacttctccaagatgtccagacaatgcaccatcaatatattct
tctgtgtttgcaagctgcatgttcatgtatccatccactgataccaagtagcctttgtactccatcccccatttcagctttaccataactggcttaccag
tgaggccattaaggaagggttttggattcagtggtaaactcatggtgcgaccgcagcctcacaacaaactgcagatcaataaaaaaaattcctccccgga
gcgtcccgtagaacacaccgctggagcaaagcgggaaatggctgccaaggcccggaagcgacaggagaaaaaaaagggggggggggnggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: