XB-CLONE-4353286
Clone Name: IMAGE:7026110
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Gene:
bcl2l12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783711
[+]
taattaatcccccccccccccgcgtttgaatacaaattccaaggagtggaaggacgtgcacccattgctgaactttgcattaaggagaactgacgaggct
gaaatttacagtttggctgcaaggaagttggacaaactagtgaaacaccagaggctgaagagcccaacggcaaccactgctccgccattcccgccaagtg
ctgaaaaaagtgatggaaagacacagttaattgaaaacaacaatgacgcaggaagcaaagtttcacatcccgaaggtaaagacgaggttattcgccagct
ggtagtgctgcttcaagagcaggcgaatgtcatcaacgataagattaacagtgatccctttttaagaaacacgctcagcaggatgtcgtatggcagcttt
tcaaagctagcagaggtctttactagccaggccgaggtaccaactgaagtagctggagtccgtgtgagccctgaactgacaaagattgccccttactatg
gagctaacccgngaaagttgctggaaatcaaacagccattgcttgtgcacccgcctgatggggctatagtaatgggcattacatggaataaggttttgta
accgggggcctacaaaaaaccaaagggggggctgggggaaaaaacatttattttctccaagaatgaaattttccccaaaactttttcaaaaatttgattt
taaaaacccccggaaaaactttttttcgggtctttaacccctcaaaaaaacccccccaaaacaacttttttaaaaggggggggt
3' EST: CF783710
[+]tttttttttttttttcaactaaaaaatatttttttaaaaatttgaagtaaaaaaaggattgacaagaatcatcccctgagattttcttaaagttgtacaa
aacattctcttgtttttgcaagtcatttaagcagccaaatgccaccagcacctaattgtggcacacatccactcttctggcttgtgtagttcccaaaaga
gccaaatagataaaaagaccactatcagcttcacaccttaagtgctgcggctctgaggtagacgagagtttctggttttaatcaatttgaaagtctggaa
tatcatcttgagaaataatgttctcccagcccccttgcttctgtagccacggtacaaacatatccatgtatgccatactatagcccatcagctggtgcac
agcatggctgttgattccagcaaccttcctggttagctccatagtaagggcaatctttgtcagttcagggctcacacggactccagctacttcagttggt
acctcggcctggctagtaaagacctctgctagctttgaaaagctgccatacgacatcctgctgagcgtgtttcttaaaaagggatcactgttaatcttat
cgttgatgacattcgcctgctcttgaagcagcactaccagctggcgaataacctcgtctttaccttcgggatgtgaaactttgcttcctgcgtcattgtt
gttttcaattaactgtgtctttcccatcacttttttcaccacttgggcgggaatggccggaaaaagtggtttggccgtttgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: