XB-CLONE-4353281
Clone Name: IMAGE:7026108
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Gene:
XB1011895
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783714 [+]
acaaagcagaatctgtcgataggagaaactttagaaaataaagtgattatatagaaatacttagcaatgccaagtacaaactgacaatagtaattaaaga
aaggggttaatgcgaattcattgttgagaatgggatatcattaactccactaggatcagctagattacagactccactagcaaagaccatgtcgagagct
caaatgcacaatggtcactgtctccaaagcactcaagaataagccaactcctaccccaaggcaaatgttaatgtactaatttgtattaaataaaaaagag
acttgactagctaaaaatccaggcaatggaaagcttttcttatgattaaacaattacaaaatgttgcagacttacccactcttccaaaattttatgcttg
gcttcatttcccttttgcaaatttctgcaagccagaatgacaagtgtaccatgtagggccaatgatcttgcggtttcaaatcctgacaacacagaaagtt
aggaaatcattaattttaataaaagaaacaaatgtaggatacagaaaaaatctactgcacattcaactgagcaacagtgtcttaaaactgacataatggg
tagttcttttctagattcaaacttttacaaaactttcctggaaccaggttattatttagaatgtttattggccgacccttccgtaaaaaaaaaaaaaaat
aaataggttcctttggtgaaaaaacactataaaaaggcccggtttcccaatttgggtcccctttcagaactaaaactttaaaaaaatttgaaaatattgt
ggcaagcaaaacggggcaaaatcaccctttaaaaggccggtataaaggcgaaagggtttatgtaacttttgagaaggaaaataaacaaaatttcagagtt
ttaaacct
3' EST: CF783713 [+]cccaacacactaccattaaggggcgtatttattatgctgtgtaaaactaattcgccgaaaaaacggagtaagatccgcgtaaaacaattacgccgatttt
acgccgtttttacgtggcgaagcctggcgatgtgtgacaaattttctcgccgttttttacacagcataataaatatgccccttagtgtataacttgaatt
tgttatttctctcaaagtacataaccttgcacttaacagcattaaagctgatttgccagtttgctgcacagatttcaaattttgtcaagtttagtcatga
agtgaacaaattaggaacaggccttttatagtgtttatcacaaagaacatattattttttttttttttactgaaggtcgccaataacattctaaataata
actggttccaggaaagtttgtaaagttttgatctagaaagaactaaccattatgtcagttttaagacactgttgctcagttgaatgtgcagtagattttt
tctgtatcctacatttgtttctttattaaaattaatgatttcttaaacttctgtgttgtcaggatttgaaaccgcaagatcattggccctacatggtaca
cttgtcattctggcttgcagaaatttgcaaaagggaaatgaaaccaagcataacattttgtaagagtgggcaagtctgcacccttttgcaattggttaca
ccataagaaaagctttcccattgcccggagtttttacactattcccgcacccttttttcactaaaacacaaattcaccacccctaacccccttgtcccct
gcggggcacgggattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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