XB-CLONE-4353265
Clone Name: IMAGE:7026102
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Gene:
rax
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783724 [+]
taattaatcccccccccccgtcattcctttcagataggtcacaaggaactgatcctctgttgttcaaggacaggctgtcagccagaaagcaaccagaagg
atctgctactcagaggcagcttagaggaatcagcctttcttaggaagatttgcagcctgctcgggctctgttgttgtggaatataccatcctgcccctgc
agtttagccttgtacagatatattaatctctttttgctaggagggaaaaaaaaactttgtacttttgaagagccagaaagcatcactttggaagtattct
ttgaaataaaatatctgcagaagtcggagcacgtggactactaagctcgatcagataaagaacttttcttggaaataatcaccgtgattgaaaacctcaa
gggtcctcgatgcacctgcacagtccttccctgatggctgacggaagcttctctctgtctggacatctgctcaggagcccaggagggaacccgtccaggc
tgcacagcatagaggccatcttgggggttttccaaagaggatagtgtcctgggctcattttcagactgagggcagtccccagaaatgccaaagaattggg
acaagaggagctctcgacactgtctacacaaaatgacgggaagaatttcatccctcaaaagagcatttaaaaaatgggccatagtggtgggctatggaga
tcctactcgggaaaagaacctcaatgaagtgcctggaacccaggctttgtccccctccataaacagaggacaaataaactttccccgatgatgatcacaa
agccccaggaaaaaatcacggaggggaacccgggaaaaacttttcccaaatttaccagcaaaccacaaaccttggagaaggggcgtttttaaaaaaaatc
gccacttaccgcggaaag
3' EST: CF783723 [+]tttttttttttttttacgtaaaaaatcttttattttcttctgtacaactttatttacaaaaaatattgtctagacgggatttcggtgctgtattgggtcg
gtgttgttatggttacatgaatgtgcattggttcatagtcccaaggaaaggcaaatccgtgcagtttaatggaagaaaagaagatttgggtacttgggga
ctaaagtcaggatatccccaaattgaaggtattcaatgtcacaaaaagtagaaacatttgagatttgttctttccttagatgattaaatcccagttgccc
aattccccctgaaacttgcctttatgaaactcattacccccccaattggcctgcacccttctttaattgtttgaagggcaaatccagtttcccaatactt
aaaactggtagccacccacccccaaaaacccggggttttttaatttggaaatgaaaaaaaaagaaatttggggggcccatccaaagggcaattttttaaa
aaggggttttacaccccccaaaatgggcataattaccccccaggggaaaccccttttggggaaaatttttttttttgggaaaaaattattttcccacaag
gtttttatgggggaaaaataacaattttccccctcccctttgggggaaaaaaaaaatatttttttattccctccccgggggggttaataaataacccccc
agggggccccccgaacatgggtaaaaaactcctgggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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