XB-CLONE-4353262
Clone Name: IMAGE:7026101
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Gene:
cat.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783726
[+]
taattaatcccccccccccccacactgcaggcagagcaccatggcaggcaataaggagaaggcacctacccagatgaaccaatggaaggaggctcggggg
tctcagaaaccaagcgtgctgaccacaggcgcgggccaccctgtaggagataagctgaatctattgaccgccggcccccgggggccactgttagtgcagg
atgtggtgttcacggatgagatggcgcacttcgaccgagagaggatcccggaaagagtggtccatgcgaaaggagcaggggcttttggctattttgaggt
cactcatgacatcacacagtactgcaaggccaacgtgtttgggaaggttgggaagagaactcccgtggccgctcgcttctccaccgtcgcgggagaagcc
gggtccccagacaccatcagggaccctcgaggatttgcagtgaagatgtacacggaggaggggaactgggacctaacgggtaacaacaccccgatcttct
tcatccgcgatgccatattattcccatcctttgtgcactctcagaagaggaacccccagactcatatgaaggacccagatatggtgtgggatttctggtc
cctgcgccccgagtctctccaccagggttctttccggttttcaaaccgtgggaatccgggacgggccaccggccccatgaaagggctaccggctcccaca
cttttttaactttgttcaactgccaaggaatgaaacccggtataactggtaaatttccaactttctaagaactggaacaagaggtgaccccccttaggtt
cttagaaggcggcccgccccactttaccccttgtcaacccttgtggccctttgt
3' EST: CF783725
[+]ggtcagtcttgaagtggaatttacagtatacagcttcatccttgcagttgacaagcttgaaagtgtgggagccgtagccattcatgtgccggtggccgtc
cggaatcccacggtctgaaaacaggaaggaaacctgatggagagactcggggcgcagggaccagaaatcccacaccatatctgggtccttcatatgagtc
tgggggttcctcttctgagagtgcacaaaggatgggaataatatggcatcgcggatgaagaagatcggggtgttgttacccgttaggtcccagttcccct
cctccgtgtacatcttcactgcaaatcctcgagggtccctgatggtgtctggggacccggcttctcccgcgacggtggagaagcgagcggccacgggagt
tctcttcccaaccttcccaaacacgttggccttgcagtactgtgtgatgtcatgagtgacctcaaaatagccaaaagcccctgctcctttcgcatggacc
actctttccgggatcctctctcggtcgaagtgcgccatctcatccgtgaacaccacatcctgcactaacagtggcccccgggggccggcggtcaatagat
tcagcttatctcctacagggtggcccgcgcctgtggtcagcacgcttggtttctgagacccccgagcctccttccattggttcatctgggtaggtgcctt
ctccttattgcctgccatggtgctctgcctgcaatgtggggggggggggggaataattaactcgagggcaaaaaaaacaagtaacttggatttcggcccc
ggaaaaaaccaagggggaattttggaccttggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: