XB-CLONE-4353258
Clone Name: IMAGE:7026099
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Gene:
dynlt5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080499 [+]
CGGCTGCTCTGTGAAGTGTTGGTCGTCTCAGCTGTAGGGATGTCAGACATAGCTAAAGACAAGGCAGCTCGTCTCCTGAAGAAAAGGGGAAGTATATCAT
CGCTGGGTAGCCATGATGTGAAACCAAGAGGCAGCTTCAGCAAAACCAAAGACTCTGTGAGCACAGTATCTTACATAGACGAACCGGGCCACCATGATGA
CATTCAGCGCCCTGCGATCCTGATGGAAAACACTTATCAGACAGGGCCTACAAAACGTTTTCCAGTAGCATCCGTAAATAATATTTTAAAGGATGTCCTT
ACAAGTTATCTTCAGGAAGAGAAATATGAAGCAGAGCTGTGCAGACAAATGACAAAGACCATCTCTGAGGTTATTAAAGCACGAGTAAAAGATCTGATGA
TCCCCAGGTATAAGATTATTGTGCTGATTTACATTGGCCAACTGAATGACCAAAGCATGCGTGTTGGAAGCAGATGCATCTGGGACCCAGCAAATGACAC
TTTTTCCTCCTACTCGTTCAAGAATAGCTCTCTGTTTGCACTTGCAAATGTCTATGGGGTTTACTATGAATAACACACGCAAATTAATTATATCCAATAC
TTTTAACAAATCTTAAGTATAGCAACTTGCAGGAACACTGAACCAAACTATATTCCTTCCCCCGGCAGCTGTGGCCAGCAACTTCCCAGATACTACATGG
CAGATGACTGAGTCCCTGGCTTACAGGGTTTTACAGTTCTGCTGGATATTTTGCATTAAGACTAGAACATGAGCATCCGCTGATCTGCTTGTTAAAGGGT
AGTTCACTTTTAATATAATGTAGACTATGACGTTCCAAGACAATTTGCATTTCATCTTCATTTTTTTGTGGTTGTTAAATTGTATAGTTTTTGGTTCAGA
AGATCTACAGTTAGGAATTTCAGCAACTATCTGAATGACAGACTGGAAGATGAATATAAGAGTACCTGAATAGAATGATGAGTAATAAATAGTAACAATA
ATAATACAGTTGTAGCCTCACAAAGCAATATATTTTTACTACCAGGATCACTGGAGGCAGAAGGCTAATTAAAAAGTTGTTAAGAGTAGGTGATTCTAAA
ACATACTAAAAGTTAATGTAAAGGTGAACTACCCATTTGAGCTTAACCTCAAATGGTTCTTTTTTGTGCATTTCTGCATCACCCCTAACCAGCAATGTGC
CCAATGTACTTACTGAAATGTTATTTATATGTACTTTTTCTATGATGCTGTAGACACTGGTATTCTAATATAATGTACAGCTGGTATTTATTTTCTGTTC
TGCAGATTTTCATTTTAAAATTCAAACCGTTACCTATCTGTTAGGGTCCCAGAGCCAGTAAGACTAATTGTAGAGTGTTTAGATAAAATAATTGAAAAAA
TATTATAACTGGAAGAGGTAATGCAATAAAGTCTAAGAGTTTGCACTGTCTAGTACACCATAGCAACATTCAATATGTAGCATTTACTGGTAACCTGTTT
TAAAGCAGACATCAAAGTGCTGTGGATTACTAGACCATTAGTGCAAATGTTGCTACATTAAATAATAACAGACTTGGCTCACAGTGAATCTCATTTGTAA
TTGTTGGGCATTGCTCCATTGTAAATTCCAAGGTAGGGAAAGACATAATGCAGAGTATAACAAATAAAAAGGAAAAATGTGAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF783728 [+]taattaatccccccccccccccggctgctctgtgaagtgttggtcgtctcagctgtagggatgtcagacatagctaaagacaaggcagctcgtctcctga
agaaaaggggaagtatatcatcgctgggtagccatgatgtgaaaccaagaggcagcttcagcaaaaccaaagactctgtgagcacagtatcttacataga
cgaaccgggccaccatgatgacattcagcgccctgcgatcctgatggaaaacacttatcagacagggcctacaaaacgttttccagtagcatccgtaaat
aatattttaaaggatgtccttacaagttatcttcaggaagagaaatatgaagcagagctgtgcagacaaatgacaaagaccatctctgaggttattaaag
cacgagtaaaagatctgatgatccccaggtataagattattgtgctgatttacattggccaactgaatgaccaaagcatgcgtgttggaagcagatgcat
ctgggacccagcaaatgacactttttcctcctactcgttcaagaatagctctctgtttgcacttgcaaatgtctatggggtttactatgaataacacacg
caaattaattatatccaatacttttaacaaatcttaagtatagcaacttgcaggaacactgaaccaaactatattccttccccccgcagctgtggccagc
aactttcccgatactacatgggcagaatgactggagtcccctggctttaaggggttttaacggttctgccggggatatttttgccatta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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