XB-CLONE-4353251
Clone Name: IMAGE:7026096
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Gene:
rps3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783732
[+]
taattaatcccccccccccccgctctttctctcccagctctgcaagatggcagtcggcaagaacaagaggctgactaaaggtggcaagaagggtgccaaa
aagaagattgtggaccccttctccaaaaaagactggtatgatgtcaaggcaccagcaatgttcaatatccgcaaccttggaaagactcttgtcaccagaa
cgcaaggaacaaaaattgcctctgatgggctgaagggtcgcgtctttgaagtaagcttggctgatctgcaaaatgacgaagtggctttccgtaaattcaa
gcttatcacagaagacgttcagggcaagaactgcctgaccaacttccatggcatggatctcactcgtgataagatgtgctccatggtcaagaaatggcag
accatgattgaagcacatgttgatgtgaagaccacagatggctacttgctccgcctgttctgtgttggattcactaaaaagcgcaacaatcaaatcagaa
agacttcctatgctcaacaccagcaggtccgtcacattcccaagaagatgatggaaattatgacccgtgaagtgcaaactaacgaccttaaagaggttgt
taataaactgattccagacagcttaggcaaagatattggaaaagggctgccagttcattttactcctcctccacgaatgttttatgtcccgtaaaagtga
aatatgcctgaaacaaacccaaaagttttgaagcttggcgcccaatcttaaatgggaacctttcccttgggcctaaggggtgggaaggaaacctgtggaa
aaaaaccactccacggggagtatctaaccaacctggggtggggcccaaaaatgttgggaaaaaacccgtggga
3' EST: CF783731
[+]ttttttttttttttggtttattttacagtttttaaatcagaattctttaactcagaccgattcctgcactgggggttcatacccatcagcacgttccact
ttggcaccagtctcgtctcctgctggttttccagctcctccaccctcgccatgaagttccattagtttgcccagctcaaactttggcttcttcagcatct
tcactttacggacataaacatcgtggagaggataaattgactggcaggccttttcaatatctttgcctatgctgtctggaatcagtttattaacaacctc
tttaaggtcgtttgtttgcacttcacgggtcataatttccatcatcttcttgcgaatttgacggacctgctggtgttgagcataggaagtctttctgatt
tgattgttgcgctttttagtgaatccaacacagaacaggcggagcaagtagccatctgtggtcttcacatcaacatgtgcttcaatcatggtctgccatt
tcttgaccatggagcacatcttatcacgagtgagatccatgccatggaagttggtcaggcagttcttgccctgaacgtcttctgtgataagcttgaattt
acggaaagccacttcgtcattttgcagatcagccaagcttacttcaaagacgcgacccttcagcccatcagaggcaatttttgttccttgcgttctggtg
acaagagtctttccaaggttgcggatatttgacattgctggtgccttgacatcataccagtctttttttggaaaaaggggtcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: