XB-CLONE-4353248
Clone Name: IMAGE:7026095
|
Gene:
lsm10
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783733 [+]
taattaatcccccccccccccgagccagacttgcgtgtcggagtgtagcagagacagggggatgagaagaactgcttgatggtatacttgaaagtttttg
gatcgcttgataacttatcctttatagtggcattgttggtgtcaacatggaagtcagccactctgtcaaagagaggaccatttcagaaaacagcctgatc
atacttcttcagggacttcatggtcatgtcaccactgttgagctccgggatgaaagctcagccactggcaccattactaatgtagatgctttcatgaaca
ttcgtctggcaaaggtcacttacagagaaccgtcgagagagagagacagagttagatgaccttttcgttacaggacgcaatatccgtttccctgcatatt
cctgaaaaaggtggatgtcattcacaccgattggaaaaccagcctgccgaaaaaatttcaaaactgtcccgcgggctttttggtgggaacaggggaggga
aaaagagttttggcaaggaacaaaaagccccctagttggaaaaaaaaatggttcttttttggaaccctaagaaaccaggtttttccttaccaaatttggc
gatttttttaggcccgcacaggcgattgccttttttctcccggttttttagngaactcacctccctctctggagtcgaataattctttgggttcagactc
tttgggccctcacaggtttaaaagagggtgcttgtgtgccctataattccgggtttatttttttgggggaaattgagtcgcccgagaagaaaaataattt
ttttgttttgttcaaaaaaaaaaaggggggtcgttcagtttaaaaataatgtttttttccctttgggggtggggaacaattnttaagtcccttttctttc
actgttaggaaagttagacatgtgtgtggaaaagtttttttgaacccaggtcatatgacaatatagagctggatgntatttgtgggggttttcctacctt
tttgtgttcctccaganagcgaggggtggtggcgtcttctcatcgggggtgtgggagaacacttggtggctcggtggttgaccccaaacgttaggggttt
tatattgtcccaccgtctattcataacgcggtgtgaaatatacgtaag
3' EST: CF783734 [+]tttttttttttttttactgtcaaattcacatttattctattataagataatgtcaccaagaaaatattatacacgctttttaaaaaatgttttggctgtc
ctatactgtatgcagccttactggcagctgcagtatctttgtggtgtctaaaggaaaagatgctgtgctaaatgcaattgtagaaactgttctaggtcaa
aagacattttttcacttgggctttcgtcttgcaaactctttcctgcctgttccaccaaagccacggacagtttgaatttttcgcagctggttttcaatcg
tgtgaatgacatccacctcttcaggaatatgcacgtaacggatattgcgtcctgtaacgaaaaggtcatctaactctgtctctctctctcgacggtctct
gtaagtgacctttgccagacgaatgttcatgaaagcatctacattagtaatggtgccagtggctgagctttcatcccggagctcaacagtggtgacatga
ccatgaagtccctgaagaagtatgatcaggctgttttctgaaatggtcctctctttgacagagtggctgacttccatgttgacacccacaaatgccacta
taaaggataagttatccagcgatccaaaaacttttcaagtatacccatcaagccagttctttctcattccccctggtctcttgctacaacttccaaaaac
gcaaagtccttggcttcggggggggggggggggggatctaaatctaattcccggaggggcgaaaaaaaaaaacaaaggtagccctttggtaatttcgcgg
ggcgcccgggtaataaaaaaacccccggagagtggcgccacattgtggggggacacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: