XB-CLONE-4353245
Clone Name: IMAGE:7026094
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Gene:
cirbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783736
[+]
taattaatcccccccccccccgacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgatgaaggaa
aactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtggtgaagga
tcgggaaagcaagagatccagaggctttggctttgtcacatttgagaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtctgtagat
ggccgtcaaatccgtgttgatcaggctggcaagtcctccaatgataggagaggtggttacagaggtggctcttctggaggcagagggttcttccgtggac
gcagaggccgatgtggtggtggagacagaggatatggtggaagcagccgctttgaaaacagaagtggaggttaccacagcagtggatccagggattacta
tggcaggtaatttttgtgaatgcctctgtaagacttccttctattttgatatttgttctcctgacaatggccctgggtctctcttgtggcaagaatcttg
ggaacctattgttgatcggtccccgaaggaccttacgggggatacttatgaccccctttttttaccccaggagtaaaatccccttccctgaactttaaga
ttccttctttcccaatgggcttgccaatttattcaaaaaaattttttggaaaacctttccacccggaaaatgttttattagcggaagataataaattcct
agcctttgttcccctcacacctctctcgttatttttctcaacaccccccccgcc
3' EST: CF783735
[+]tttttttttttttttcttttcacattggtttttttaataggcttcgtgaagccaatcaaaagtagaatacaattccttagccacttcagtacaatagttg
catgtttagtaaaaaaaggacaatataaacaaacattttaagaacttcttctgatattgttactggagatgcgaacactattcactttgtgtatcaagga
ggtgtagccactatatacagcgcacccaagctctgccttgccttgttacactaaaggaatgcaatttaagtggactcataaaattaacttttcatgtaac
caaaaaatacagcagcttaaagttctttaaacaatgctacaggaagacaggcctagcagtctgctactaatacaggtgcttctctgcccatcaaccaaca
gcacccatccgcttaggaagttacttcctagcactacttaaccagaagggggagcagccccacctgatcagcttgaatggaacagcatagtaggatagga
atatccttgcagaacacagtaactttaagaatgagattaggtgtccaaagataaagcctttgaaacacagctgtcccacttattagagaagctgtcaggc
taactaggtctattaaaaaaaaaagtgaacacaagtagatatactacacaaacgattcggcgaagctccaaaaatctttataaatacagccattgggagg
acgatcttgagtcaagaatggattttactcttgtgtagtatagctgtcataactatccctggtaggatccttccggaacgaatcaccataactttcat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: