XB-CLONE-4353242
Clone Name: IMAGE:7026093
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Gene:
psmc2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080137 [+]
GAGTACAAGGTTCTTGCTGTGTAATAACCGGCAGGTTCGGAATTGTTTCGCTCTGACAGTTCTTGTGCTTTGTGGATAGACATCATGCCTGACTATCTAG
GAGCGGACCAGCGGAAGACAAAAGAAGAAGAAAAGGAGGACAAACCTATCAGATCTCTTGATGAAGGAGATATTGCATTGCTGAAAACCTATGGACAAAG
CACTTATTCAAGGCAGATCAAGCAGGTAGAGGATGACATACAGCAACTGCTTAAGAAAATAAATGAGCTTACAGGTATTAAGGAGTCTGATACAGGACTT
GCTCCTCCAGCACTGTGGGATTTGGCTGCAGATAAACAGACACTTCAGAGCGAGCAACCACTTCAAGTGGCAAGATGTACAAAAATAATAAATGCAGACT
CAGAGGACCCAAAATACATTATTAATGTAAAACAGTTCGCTAAGTTTGTGGTAGACCTCAGTGATCAAGTTGCTCCTACTGACATTGAAGAGGGTATGAG
AGTCGGTGTTGACAGGAACAAATACCAGATTCATATCCCTCTTCCTCCAAAGATTGATCCCACTGTAACAATGATGCAGGTAGAGGAGAAACCTGATGTA
ACATACAGTGATGTTGGAGGCTGCAAGGAACAAATAGAAAAATTGCGAGAAGTTGTGGAAACACCACTGCTTCATCCGGAGAGGTTTGTTAACCTTGGTA
TTGAGCCTCCCAAAGGAGTACTGCTGTTTGGACCTCCTGGAACTGGCAAAACGTTGTGTGCACGTGCTGTAGCCAACAGAACAGATGCCTGCTTCATCCG
AGTCATTGGATCTGAGCTTGTGCAGAAATACGTGGGAGAGGGAGCCCGTATGGTGCGTGAGCTATTTGAGATGGCCAGAACCAAAAAAGCATGTCTTATT
TTCTTTGATGAAATTGATGCCATTGGTGGGGCTCGTTTTGATGATGGAGCTGGAGGTGATAACGAAGTCCAGCGCACTATGCTAGAACTTATCAACCAGT
TGGACGGCTTTGATCCCAGAGGAAACATTAAAGTGTTAATGGCCACTAACAGACCAGACACTTTGGATGCTGCTCTTATGAGACCTGGAAGACTGGACAG
AAAAATTGAATTTAGTTTGCCCGATCTGGAGGGCCGTACGCACATATTTAAGATTCATGCACGTTCTATGAGTGTCGAGAGAGATATAAGATTCGAACTT
CTGGCTCGGCTATGTCCTAACAGTACAGGAGCCGAAATTCGTAGCGTGTGCACAGAGGCTGGAATGTTCGCTATTCGAGCTCGCCGTAAAGTCGCAACTG
AGAAAGATTTCTTAGAAGCTGTGAACAAGGTGATCAAGTCGTATGCAAAGTTCAGCGCTACCCCAAGATACATGACTTACAACTAATTCCAGCAGCTACT
GTTTTTGGCACCATTATCTGTTATGTAGAACAATATTGTGTTGGAATATAAATAAATAGTAAAAACAAAAAAAAAAAAAAAA
5' EST: CF783737 [+]taattaatcccccccccccccgagtacaaggttcttgctgtgtaataaccggcaggttcggaattgtttcgctctgacagttcttgtgctttgtggatag
acatcatgcctgactatctaggagcggaccagcggaagacaaaagaagaagaaaaggaggacaaacctatcagatctcttgatgaaggagatattgcatt
gctgaaaacctatggacaaagcacttattcaaggcagatcaagcaggtagaggatgacatacagcaactgcttaagaaaataaatgagcttacaggtatt
aaggagtctgatacaggacttgctcctccagcactgtgggatttggctgcagataaacagacacttcttagcgagcaaccacttcaagtggcaagatgta
caaaaataataaatgcagactcagaggacccaaaatacattattaatgtaaaacagttcgcttaaatttgtgggtagaccctcagggatcaaagttgcct
ccttactggaaatttgaaaaaagggtaatgtaagaaatcaggttggtttgtaccagggaaaccataaatgacccagggaatttccggtatatcccccctt
ccctttttcctcctcccctaaaaaagtaattgtagaaattctccctcaacgcttggtttataatacccacatatgtggaaaatggccccaaacgggtttt
taggaacgcgggaagcaaataaaacccccttcggaatttggtttaatacctattattcaccaaattggggcataggtttttgtgggaaagaggtccttgc
gcttaatgtggaatattccaaaattttaggaacaaaaaaatattgggccgtaaagaaaagtttttgttgggagataaaacatacgcacgcttgcccgatt
tctaatcgccgggagaaaaggggttttggttttaaacacctattgggtttaatttggaaagcctcttcccctaataagggagtacactcggcctggattt
tgggaacctttccttgtggaactctgggcaaaaaaacatttggtggggacttgttgcgtgtaaagccga
3' EST: CF783738 [+]tttttttttttttttgtttttactatttatttatattccaacacaatattgttctacataacagataatggtgccaaaaacagtagctgctggaattagt
tgtaagtcatgtatcttggggtagcgctgaactttgcatacgacttgatcaccttgttcacagcttctaagaaatctttctcagttgcgactttacggcg
agctcgaatagcgaacattccagcctctgtgcacacgctacgaatttcggctcctgtactgttaggacatagccgagccagaagttcgaatcttatatct
ctctcgacactcatagaacgtgcatgaatcttaaatatgtgcgtacggccctccagatcgggcaaactaaattcaatttttctgtccagtcttccaggtc
tcataagagcagcatccaaagtgtctggtctgttagtggccattaacactttaatgtttcctctgggatcaaagccgtccaactggttgataagttctag
catagtgcgctggacttcgttatcacctccagctccatcatcaaaacgagccccaccaatggcatcaatttcatcaaagaaaataagacatgcttttttg
gttctggccatctcaaatagctcacgcaccatacgggctccctctcccacgtatttctgcacaagctcagatccaatgactcggatgaagcaggcatctg
ttctgttggctacagcacgtgcacacaacgttttgccagttccaggaggtctaaacagcagtacttctttggggaggctcaataccaaggttaacaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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