XB-CLONE-4353239
Clone Name: IMAGE:7026092
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Gene:
rpl6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783740
[+]
taattaatcccccccccccccgcttctttttccttacagccaagatggcaggagaacagcaacctgttaagaaggccaggaagcaccattgcagtcgcaa
ccccgtactggcccggggtattgggaagtattcccgttctgccatgtatgctaggaaggccctttataagcgcaagttcaaggctccacagacaaagatt
gaaaagaaaaagcaaaaggctctagtaactgtaaccaagacagttggtggagataagaatggtgggacccgtgtggtgaagttgcgtaagatgccccgtt
attaccctactgaagatgttccacgcaaattgttgagtcatggaaagaagcctttcagccagcacaaacgcaagctgcggtcttcaatcactcctggaac
agttttgattcttcttaatggacgccacaggggaaagcgcgttgtcttcttgaaacaacttcaaactggccttctgcttgtaacaggacccttggctata
aacagggttcctctaaggagggctcaccaaaagtttgccattgccacctctactaagatcgatatctcaagtgtcaagattcccaagcatctgacagact
cctactttaagaagaagaagctgcgcaaacctaagcatcaggagggtgagatctttgacactgagaaagagaaatatgcagttacacaacagcgcaaagc
agatcagaaatttgtggactctcagctcctgcctcttctcaaaaaagagcctcagcttcgtgggtacttgcgttctgcattncttcctgtcaatggggat
gtacccctcataaactgggtctt
3' EST: CF783739
[+]tttttttttttttttgttttctcacaagatactttttaataagcccactttttaaaagaccagtttatgagggtacatcccatttgacaggaagaatgca
gaacgcaagtacccacgaagctgaggctcttttttgagaagaggcaggagctgagagtccacaaatttctgatctgctttgcgctgttgtgtaactgcat
atttctctttctcagtgtcaaagatctcaccctcctgatgcttaggtttgcgcagcttcttcttcttaaagtaggagtctgtcagatgcttgggaatctt
gacacttgagatatcgatcttagtagaggtggcaatggcaaacttttggtgagccctccttagaggaaccctgtttatagccaagggtcctgttacaagc
agaaggccagtttgaagttgtttcaagaagacaacgcgctttcccctgtggcgtccattaagaagaatcaaaactgttccaggagtgattgaagaccgca
gcttgcgtttgtgctggctgaaaggcttcttttcatgactcaacaatttgcgtggaacatcttcagtagggtaataacggggcatcttacgcaacttcac
cacacgggtcccaccattcttatctccaccaactgtcttggttacagttactagagccttttgctttttcttttcaatctttgtctgtggagccttgaac
ttgcgcttataaaggggccttcctagcatacatggcagaacgggaatacttcccaataccccggccccgtacggggttgcgactgcaatggtgcttcctg
gcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: