XB-CLONE-4353229
Clone Name: IMAGE:7026087
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Gene:
dhrs3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080136
[+]
CCGAGGAGCAAAGCAGAAGCAGGAGTCGGGCAGCGCTCACATCTCCGCTCCAGCCCTGCAGCACACACGGGGCATATGACATTGAACAAAACTTCCATTC
AGTGCGCTCCCCTGTGAAAGTGCTGCCAAGTGCCTGGCTCTGCTGCGGGCCATTTCCTGACTAAGAATAGCAGAGAGCAGCTTACTGGGGACTCCTCAAT
CTGTCAGCAACGCGGGGGAGTCTCAGCGCTGCACCGGGCAAACCATTGCTGCACCGACAGGGGGGAACCCGCAGCTCGCCTTGCCCCAGGACAGGGCAAC
TATTGCAAGGGACTGTGCCCAGCAGCGCGGCTCTGGTATTCATTGACTTGTCATTTGGCCGGGGCTGTACGAGTGACAGGGCTGTGTCCCTTCAGGAGCT
GACAGACCGCTCCGTGCCCGGGGAACTTGGGGAGAAAGTTGCGGCTTGGGTGCAGCAGCCATGGATATGCGCTCAGTGGGCAGGCTGCTGCTGTTCCCCG
TACAGATGCTATTCGCCATTCTAAAGGCTGCTGCGAACTTGCTGATGCCCACTAGGCTGAGGGACCTGAGCGGGGACACTGTGCTCATCACTGGCGGAGG
CAGGGGGATCGGCCGCCACCTCGCTAGGGAGTTCGCAAAGCAAAACGCTAAAAAGATAATTCTGTGGGGCCGGACAGAGAGGTGCCTTAAAGAAACTACT
GAAGAAATAAAGCAAATGGGAACGGATTGTTCATACTTTGTATGCGATGTTGGTAATCGTGAGGAAGTCTACCAGCAAGCAAAAGCTGTCAGAGAAAAGG
TTGGAGATGTAACCATTTTGGTGAACAATGCAGCGGTTGTACATGGCAAGAGTCTCATGGACAGTGACGATGATGCTCTCTTGAAGTCCCAACACATTAA
CACACTAGGACAATTTTGGACTACAAAAGCTTTCCTGCCACGTATGCTGGAACTTCAGAATGGCCACATTGTTTGTATAAACTCTGTGCTGGCCTTATCT
GCAATCCCTGGTGCCATTGACTATTGCACTTCCAAAGCTTCCTCCTTTGCCTTCATGGAAAGCTTGACCTTGGGCCTTTTGGACTGCCCAGGAGTAAATG
CTACAACAGTACTGCCATTCCACACCAACACAGAGATGTTTCAGGGAATGAGAGTAAGGTTTCCTAACCTTTTCCCACCTTTGAAGCCTGAAACAGTAGC
TACAAAAACAGTGGAGGCTGTTCAAAAGAATAAAGCTTTTCTTCTGCTTCCATGGACGATGCACGCACTTGTGATCTTAAAAAGCATCCTTCCTCAATCA
GCACTTGAAGAAATACACAGATTTTCAGGAGCTTACACCTGTATGAACACTTTCAAAGGGAGGACATAGACACCATGAAAGACTACTGTGCGCAATTAGG
CACAAGCAATCCTAAGAAGACTGTGGAGCCGAGTCTCTACTTTCATGCCCGGTCTTATGACTGATGCTCTTGCGGCATATGGTTCTTCCAGGTCATTTAT
CCTGTAACGTGGCCTTTGGGCAGGCCCCAATAATGCTGAACTCTATGGACTCTGGAGCACGTCAAGTGAATTTTGTTTTCCTTTCTTTTAGGGATTTAGA
GTTTTTATTGTTTAACTCTGCTGGTAGCAAGAACAGATTCAAATTTACTTTTTTTAATCTGCTTTTAGCAATTGTTTAAGCAACAGTTGCATTGTGTTGT
AGTGCTAAATACAGACCAATACTGCTTCTAATCCAGTGAAAATGTTGGAAATCATTAGCGAGTTCACCCCCCGCATTGTATTTTCTCAGGATAATTAACA
GAGCATATTGGTATACTTGCCTGAAAAAACACAATACTTAAATTTAAGTAAGGTTTTAATATATTTTGTTAAGCTATTCAATTTTAAATGGCTTCAGATT
TTGTGTTTCTACAAAGGGCTTGAAAAAAAAATCAGGTTTTGTACAGCTTTGTTTTTAAGGTGTATTTAAAAGCATTAAAGGGGATATAGCTTGGCTCCCT
TCCTTAAATGCTTTGTTGACATTTAACTGAAACCAGTAACTGTAACCAAAATAATATACATTATAATGTGTATGTTCACTTGCGCTGAGAGAAAAACTGG
TCTACCATTTTATTCGTATCTTGCAACGCTCACAGTAAAATGTTTGGAAAAGAGAAAAAAAAAAAAAAAAA
5' EST: CF783745
[+]taattaatcccccccccccccccgaggagcaaagcagaagcaggagtcgggcagcgctcacatctccgctccagccctgcagcacacacggggcatatga
cattgaacaaaacttccattcagtgcgctcccctgtgaaagtgctgccaagtgcctggctctgctgcgggccatttcctgactaagaatagcagagagca
gcttactggggactcctcaatctgtcagcaacgcgggggagtctcagcgctgcaccgggcaaaccattgctgcaccgacaggggggaacccgcagctcgc
cttgccccaggacagggcaactattgcaagggactgtgcccagcagcgcggctctggtattcattgacttgtcatttggccggggctgtacgagtgacag
ggctgtgtcccttcaggagctgacagaccgctccgtgcccggggaacttggggagaaagttgcggcttgggtgcagcagccatggatatgcgctcagtgg
gcaggctgctgctgttccccgtacagatgctattcgccattctaaaggctgctgcgaacttgctgatgcccactaggctgagggacctgagcggggacac
tgtgctcatcactggcggaggcagggggatcggccgccacctcgctagggagttcgccaagcaaaacgctaaaaagataattctgtgggggcggacaaag
aagtgccttaaagaaactactgaagaaataaagcaaatgggaacggaattgttcatacttttgtatgcgaaagtttggtaaccgtggaggaagtctacca
ggccc
3' EST: CF783746
[+]ttttttttttttttttctcttttccaacattttactgtgagcgttgcaagatacgaataaaatggtagaccagtttttctctcagcgcaagtgaacatac
acattataatgtatattattttggttacagttactggtttcagttaaatgtcaacaaagcatttaaggaagggagccaagctatatcccctttaatgctt
ttaaatacaccttaaaaacaaagctgtacaaaacctgatttttttttcaagccctttgtagaaacacaaaatctgaagccatttaaaattgaatagctta
acaaaatatattaaaaccttacttaaatttaagtattgtgttttttcaggcaagtataccaatatgctctgttaattatcctgagaaaatacaatgcggg
gggtgaactcgctaatgatttccaacattttcactggattagaagcagtattggtctgtatttagcactacaacacaatgcaactgttgcttaaacaatt
gctaaaagcagattaaaaaaagtaaatttgaatctgttcttgctaccagcagagttaaacaataaaaactctaaatccctaaaagaaaggaaaacaaaat
tcacttgacgtgctccagagtccatagagttcagcattattggggcctgcccaaaggccacgttacaggataaatgacctggaagaaacatatgccgcaa
gagcatcagtcataggaccggggcatggaaagtagagactcggcttccacaatcctcttaaggattgccttgtgcctaattggcgcacaagaaatccttt
cctgggnggcctatgtccctccccta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: