XB-CLONE-4353226
Clone Name: IMAGE:7026086
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Gene:
nat8.4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783748 [+]
taattaatcccccccccccccgatacctgagctcccagttctgttgggatatgtgcccgggtctcccactcctgcctcagggggacacaaagcaattact
ggaggaggccattacttagccggagcatcatggccaatgtttccataaggaaatacaaaaccagtgactatgagactgcccgcttcttgtttgccgaagg
aactaaagagcatctcccagcagcctgtatgtacacgctgacgacacctcgattttattttattacctttgtggcatgtaccagtgtattcatgggcacc
ggttcctacgttctggcccttaccagccttgtcgccctgttggctgctggttggtatggcttgtactctgaattccatgggcttgcaagccggtttctgc
gcaaggatatgcttgatattgagaagtcctacatgatgagtgaaaatgcctgtttctgggtggcagagatggacgggaaggttgtgggcacagtgggaac
ccaaccatctacagaggcagatgatgagctgttgctgcaacgtacatctgttgcccgggactatcgccagctgcgaattggcacaaaattgtgccagacg
gtcattgattttgcccggcagcggggcttcaacgctgtgtgtctggaaacagccaatatacaacgtgctgcgacgaacttgtatgaaagagttggcttca
agaaatcccgtgttgagatcctcccatcacttgttcatcaatatacatccttttacgggttgctttatttacagataccgacattcaaatcattaagcaa
atccccagtggttttaataaatgtatggt
3' EST: CF783747 [+]tttttttttttttttcgagtttaataatagactgtttaatagcaagacacaggaagcaatgtcacatggaatatcacaagtagacgtattgtgaaacgtt
aagaccaaaatccaaagaacaaatgatttccagctttgcctgaaggtcttttttgcactttgtatacattccagccgacatcacgtgggcagagacaaca
ccggccaatactaaattggttcctgtttatttttcttttaacttttatattaaaagtaaaacatctcaaggggcttctggtggaaccccttccatgaaga
ctttccaaaatgcatttagttataacctgataataatctgtaattatctgaactgattcatcttttccagtctttagggtagagtcctgcattgggttgg
ataccagaaacaccccgattccctaaacatacaggtggtctgcaggttccctgcatgggtatgtggacggattgtaattgcatttccccccatctccaaa
gtcaaaagtcatttttccccctgacctggttggtccccctcaggagtgatgcaacttccacccaccaatgatgtctctttccggtttgtccccagattgg
gttaaatgctttgggtagtccggcttaagttgtgtatcggggacagggcacttattagcgggtcaggttgtgcgttatgggggccccggtttggggcaca
ggtctggcccaactggaaccccttgcaggaactctacttttatgaattcctcccccattttggatcaacctgaattgtgtacccttaccattataaacca
cctggaattggctaaggattttgaaggtcggaacccggaaaataaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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