XB-CLONE-4353218
Clone Name: IMAGE:7026083
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Gene:
vangl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783752 [+]
taattaatcccccccccccccttttttttttttgtattttccagccattctgctcaataaccactaaccttgtggcactattattttattaccctctttg
tacaaaatttttctacttttgttgagacacttcgtaaaaaaaaaataaaaaaataaggcccccattttgtaattgcggaacatgacgtatttgctcttct
ggactggattcccgactccgcctgcagaagggcgtggccttgtccttgctgcccactgtgaatgggtgagaggatctgaaggcgtggcttgcattgccta
tgcagttcaatcatcgacaccgaccagggaaaacaaacaaaaatgactattgaaccggctgttacattccaggcccatgattattctggattgttcttca
ctacacaatgcactagcaaatcagcatttgtttacatcacacgtggccagatttatcgacggaattgttattgggtatcgggtattcaatttgaaattcc
ctgacagtgggaattcctagtaccgcgctttttatttaagccaagggtcgttagtactgttttttaaaaaggcgtttttgttgatttccccatggcatta
attctctggaaagttccaaattgcgggggggggggggggggggggaaatatatctctccatattgacttatcacactcaccggagatacaaatactgttg
tagagaggtcggggtaagtccgtggggcagtcttgacgccttttttatgtgccctctcactataggtttgtggctgtccggtggtattttgttaatgttg
ttgg
3' EST: CF783753 [+]tttttttttttttttggtgtaaaaacaactttattattgacgtccttcagtggtgagaggctacaaacattacaagtcttcggttccactcgcaaagtac
agtaacaatctgaacctgaacagtaaacagacaggcggaatttttatttccccccccccccaaccccccgnaatttggaactttccagagaattaatgcc
atggggaaatcaacaaaaacgcctttttaaaaaacagtactaacgacccttggcttaaataaaaagcgcggtactaggaattcccactgtcagggaattt
caaattgaatacccgatacccaataacaattccgtcgataaatctggccacgtgtgatgtaaacaaatgctgatttgctagtgcattgtgtagtgaagaa
caatccagaataatcatgggcctggaatgtaacagccggttcaatagtcatttttgtttgttttccctggtcggtgtcgatgattgaactgcataggcaa
tgcaagccacgccttcagatcctctcacccattcacagtgggcagcaaggacaaggccacgcccttctgcaggcggagtcgggaatccagtccagaagag
caaatacgtcatgttccgcaattacaaaatgggggccttatttttttattttttttttacgaagtgtctcaacaaaagtagaaaaattttgtacaaagag
gggaataaaataaaagtgccccaaggttagtggttattgaagcaaaagggctgggaaaattccaaaaaaaaaaagggggggggggggggaattaaattta
aattccgggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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