XB-CLONE-4353215
Clone Name: IMAGE:7026082
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Gene:
myl6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783755 [+]
taattaatccccccccccccccccgacattccggaagggagaagacgcgtcaagatgtgcgactactccgacgaccagattgccgactacaaggagtcct
tccaactttttgaccgtgtcggcgatggcaaaatcctcttcggccagtgtggggacgtcatgcgggcactggggcagaaccccactaatgctgaggtgat
gaaggttttgggaaacccaaagcctgaagacatgaacatcaagacacttgacttcgagcaattcctccccatgatgcaaacagttgccaagaacagggat
gtacctggattggaggacatcatagaagggctaagagtcttcgacaaggagggaaatggcacagttatgggctccgagttgcgtcatgtcctggtttccc
tcggagagaagatgacagatgatgaagtagagacccttttaagtaatcatgaggatgctaatggttgcattaattatgaagaacttatccgggcgatatt
gaacggctgaagcttctcctttagtttacctctgcccagtggggatgaagtgtgggggggggaagttagttagcaatttattcacgccctttccctagag
agggagacaggctatatagttaagtgcccttttctgcttccctcctttatcccaatcagtcaccccctttcttcttcgcaggtgggcaaaaatcggtaat
cctttttttcccggaaacattcccaaggggagggtcctcccgcttacagcccttacatggaaaaccccgcgtaagaaattgtgggcacaaaaaatatttt
tccgtatgcacccccccc
3' EST: CF783754 [+]tttttttttttttttggaaaaagtcactcagtttatttgatgttggataggggaaaaaatagaaatttgaaataatttatctttctctgcacactacagc
attaaggaagcatgggggggggggggtgatatgaaaaattattttgccacaatttctaccgatgttcatttaagctgtagctgtgggcctcccttgtgat
gttacagaaaaaatgaatacgaattttgccacttgcgagaagaagggggtgactgatgggataaggaggaagcagaaaaggcacttactatatagcctgt
cttcctctctagtgaagggcttgaataaattgctaactaacttcccccccacacttcatccccactgggcagaggtaaactaaaggagaagcttcagccg
ttcaatatcgcccggataagttcttcataattaatgcaaccattagcatcctcatgattacttaaaagggtctctacttcatcatctgtcatcttctctc
cgagggaaaccaggacatgacgcaactcggagcccataactgtgccatttccctccttgtcgaagactcttagcccttctatgatgtcctccaatccagg
tacatccctgttcttggcaactgtttgcatcatggggaggaattgctccaagtcaagtgtcctgatgttcatgtcttcaggcttttgggtttcccaaaac
ctttcatcccctcagcattaggggggtttctgccccaattgcccccctgaaatcccccacactgggcccaaaaaagatttttgccttccccccact
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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