XB-CLONE-4353209
Clone Name: IMAGE:7026080
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Gene:
rrp7a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783759
[+]
taattaatcccccccccccccaagatggcgccggccaggcagtgttccacgctcgcagcccctgcaggttacacagccgtcccagtgaaattctcagaag
aacggagaacacatcactatttgtacgttaaggagcacaaagtgagagaagaattagatcaaacacggccacaaaaccgtacgctgtttgtccttaacgt
ccctccgtactgcacaaaggaaagtctgttccagatattcacatcctgctctcccgtggagttggtagagttacaggataaaccagggccttctgagccg
cagtctaacacagtctcacaacactttggccggaggcaactcaagggctttaaagttgcatatgttgtatttaaacacccgtccggagttcaggccttta
aatcattaaaagttaagaaaccaattgtgttgtcagaagtcagccattctataaaaaccggaattaacaagtgggtagatgattatcgggcgtctctcac
gggtactgcagagctgcaagctgaagtagatgaatttatgaagggatacgacgagagggtggcagaggaagaggaaaaggctaatgaagaagagggagtc
cctgatgaagaaggttgggtgaaggtgacacggaagggccggcgtccaggcttagcaagaacagaggctgtaaatatccgggtaacagaacgagaaaaga
ggaagagagcccaaaaggaactcctcaatttctatgcttggcagcacagagagagcaagcgagagcacctagctgagctacgtaagaagtttgaagaaga
caagcagaaagatgc
3' EST: CF783758
[+]tttttttttttttttcttttttttttttttttttttttttttttttttaaattaaaaattgggtttttatttccacttgagttttgtgtcacagtaagag
tacaaaaatgtccctaaacttttcaaacccagattccaaagggcaacctttgcatccatattagtcatgatggtggtggacttcctcttgattgaggcat
ccacacaagatgtactatggctggattatccacagtggcctacatcaaaaagggcggaatttcctctgtgcacgcatcagggcaatcttctgcttgtcct
cttcaaacttcttacgtagctcagctaggtgctctcgcttgctctctctgtgctgccaagcataaaaattgaggagttccttttgggctctcttcctctt
ttctcgttctgttacccggatatttacagcctctgttcttgctaagcctggacgccggcccttccgtgtcaccttcacccaaccttcttcatcagggact
ccctcttcttcattagccttttcctcttcctctgccaccctctcgtcgtatcccttcataaattcatctacttcagcttgcagctctgcagtacccgtga
gagacgcccgataatcatctacccacttgttaattccggtttttaaagaatggctgacttctgacaaaccaaattggtttcttaacttttaatgatttaa
agccctgaaatcccggaggggtgtttaaatacaacatatgcaactttaaagcccttggaattgcctcccgcccaaagtgtttgtgagactgtgtttaaac
tgcggcctcaaaaagccccggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: