XB-CLONE-4353206
Clone Name: IMAGE:7026078
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Gene:
cidea
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080498
[+]
AGTGAGAGAGCCCGGGGTATATGCTGAGCTGACTGACAAGATGCAGGGCGCTCTGGACTACGCTAATGCCCTGTCCCCCAAGTCCCTCATAAGATCTGTT
ACTAATGTTGGTACATCACTGACACGAAGAGTGTTATTTCCGCCTCTTCCTGAGCCTCCACAAAGACCTTTCCGAGTCTCAAACTCTGATCGCAGCAGCA
AGAAGGGGATTGTGGCAGGTACTTTAAAGGAGCTCATTGAAAAGGCCAGTGAAACCCTCTTTATACACTCTGACCTGGTAACCCTTGTGTTGGAGGAGGA
TGGCACAGTAGTGGACACAGAAGACTTCTTCCAGTCATTGGAGGATAACACACAGTTTTTGCTGTTAGAGGCAAAACAGAAATGGACACAAGAGAGAAAC
TCTAAACGTGCAGTGCAGCATGAGAAAAAGACTGGAATTGCAAACCTGACGTTCGATTTGTATAAACTGAACCCAAAGGATTTTGGTGGCTGCCTAAACA
TTAAAGCAACTTTTTATGAGATCTATTCCATATCCTGGGACATGCAGTTCCTGGGTGCTAAGAAGGTGTTGAGGCAGCTAATCCGTGGACTTTCCTATCT
GGCCCAGGTAACTGGCCATTTGCTTCTGTACGGTGGCTCTTTCGTAATGCAATGGACTGAAAACTTAGAAATGGAGAGGCCAATTCCAAAGTCTCAGCGC
TCCTAACACCCAAGCAGAACCTTGTACATGGCCTCATTTAACCCAGATGAATCTCACTTGTGACAAGTTCCGGCCAGTGGAAGCGATATACTCCATGTCT
TTCTTATAAACTAATGCATCAGTCCATCATTTACCAAATTTACCCGTTTACATGTTTTGTATTTCTGTCCTCTATAAACCTTTTAACTTTGTTTTTGAGC
ATATTTTTTGTATATTGTAGTGCCTGCTCTAAACAGTTAAAACTAACTTATAAAACTCTTTTGTCTAAGCAAGTCATTGGTCAGATCTGTGAGTAAGAAT
GCAAGAGATTTATCATTGGGGACTGAGGAAATGAAAGTATTGTTTCTATGTTTCTACTGCAAAACCATGTAATGTATAACTCGTTTACCGAAAGGACAAA
TGCCTCCATAACTTATTGTGTCAGTTGTTTACAGAATTCGACAGTATATAGAAGAGCCTGCTTTTTCTTATGTTTCTCTTCATTATCTGTTGTATTTTTG
AAAGGGAATCAATTGATTTCAATAGTGCGGTGCTAGGAAGCTTCAGTTGCTGCTACATGGCATAACCTCATGTGTTCATTGCTTAAAATATTTATTTAAC
ATAGCTGTAATTACAGTGGAATTTTAGAGATATTTTTGTTTCATTTTAAATAAAAAGCTCCACTCCAAAAGAGCATAAACAAACTATGTATGCCTGATAT
ATCACCTAATTAACAGACTATCATAAAAGGCATCCTTATAAATATATGATCTGTGTGGGTTATCAAGATCATTGAGATAAAAAATCCATCCATTGTCACA
GACTTGCTTCATATCTCATATGTGGCCCTTGGTTTCTACTTTTCCAGTCAGTAATTGGTTTAAACCTTGGATGTGTCAGATGTACAGAAGAGATTTCTTC
TCATCCAACAGAATAAGCTACTTGATCACCTCATATTGTGCTTTAATGCAAAGCTAACTTTTAACAATCTTTTTTTTTAGTCCTTGTTTACATTTTAATT
GCTGTATATCTATATAAATTGTTTAAATGAGCACTGGATATATGTTCGGTATAAGATGTAAAGGTTTACTAAAATGCATGATTTGATATGGGAATTATAT
AAAGGAAAAATTCCAATAAATCCTTTCACAATTTTCCTTAAAAAAAAGAAAAAAA
5' EST: CF783761
[+]taattaatcccccccccccccagtgagagagcccggggtatatgctgagctgactgacaagatgcagggcgctctggactacgctaatgccctgtccccc
aagtccctcataagatctgttactaatgttggtacatcactgacacgaagagtgttatttccgcctcttcctgagcctccacaaagacctttccgagtct
caaactctgatcgcagcagcaagaaggggattgtggcaggtactttaaaggagctcattgaaaaggccagtgaaaccctctttatacactctgacctggt
aacccttgtgttggaggaggatggcacagtagtggacacagaagacttcttccagtcattggaggataacacacagtttttgctgttagaggcaaaacag
aaatggacacaagagagaaactctaaacgtgcagtgcagcatgagaaaaagactggaattgcaaacctgacgttcgatttgtataaactgaacccaaagg
attttggtggctgcctaaacattaaagcaactttttatgagatctattccatatcctgggacatgcagttcctgggtgctaagaaggtgttgaggcagct
aatccgtggactttcctatctggcccaggtaactggccatttgcttctgtacggtggctctttcgtaatgcaatggactgaaaacttagaaatggagagg
ccaattccaaagtctcagcgctccctaacaccaagcagaaccttgtacatggcctcatttaacccagatgaatctcacttgtgaacaagttccgggccaa
3' EST: CF783760
[+]ttttttcttttttttaaggaaaattgtgaaaggattattggaatttttcctttatataattcccatatcaaatcatgcattttagtaaacctttacatct
tataccgaacatatatccagtgctcatttaaacaatttatatagatatacagcaattaaaatgtaaacaaggactaaaaaaaaagattgttaaaagttag
ctttgcattaaagcacaatatgaggtgatcaagtagcttattctgttggatgagaagaaatctcttctgtacatctgacacatccaaggtttaaaccaat
tactgactggaaaagtagaaaccaagggccacatatgagatatgaagcaagtctgtgacaatggatggattttttatctcaatgatcttgataacccaca
cagatcatatatttataaggatgccttttatgatagtctgttaattaggtgatatatcaggcatacatagtttgtttatgctcttttggagtggagcttt
ttatttaaaatgaaacaaaaatatctctaaaattccactgtaattacagctatgttaaataaatattttaagcaatgaacacatgaggttatgccatgta
gcagcaactgaagcttcctagcaccgcactattgaaatcaattgattccctttcaaaaatacaacagataatgaagagaaacataagaaaaagcaggctc
ttctatatactgtcgaattctngtaaacactgacacaataagttatggaggcatttgtccctttcggtaaacgagttatacattacat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: