XB-CLONE-4353200
Clone Name: IMAGE:7026076
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Gene:
ndufv1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783765 [+]
taattaatcccccccccccaacccccccgatccccaacaccctgagaaggaacaagatgaaagccattatcctgtgcgctctgctagccaccgtcctgat
tcaggctgtggtcagtgatgatgtggagcaatctgagaatgcagttattggagctgtggatcaaactgagcaaggggtcgtaaaagctgcaggtcaaact
gagcaagagcataagatccgtcgccgccgtcgcttgcctgcttattaagaccctatggcaactggcaagtttcaaggggttgacagcattgaacattgca
atcttttgatccgatttcttgggtctgagtcctcgtcgagtcctgggtgttcttgaccctactgcccccctaaactccgatttttccccctttttttcct
gctccatttccttcacccctgctttgtgcttccccccccacctattcccccctcactcccattaactcttgggtcgcttttcccaaatatccataatctc
cctatttaatttcccgttgtttttgaatcatattttcaacatcattgttttttcgccccttatttttttttacagaccaaggaccgcctccaccacaata
tagcgtgggaattcctttcgggctaccatgtgggagtcccccaaacctctttggtatttgttcgcagccgaagatgtttttatacaactgggggtttttc
cacaaatttccccgctattatgtttgccccctattccccccacttttccgcccaattacacaagagcctcccttcccgcttcgtgtctgtgataccattt
taatttactctcttaattatgtataatacacaaatcctcacttctatgtgaccacgccttttattcctctttgtttcccctggtttcgcggcctaatata
aacctcatctttttttgcccccaccccccctttttattttcacccccccattcattatgcaaatattcacccccccctttgtacctatgtggcccccact
ttgtaatttg
3' EST: CF783764 [+]tttttttttttttttctttttttttttttttttttttttttttttttttattagcagccaaaatttattgggatagggtattgtatgtcggggaaaagcc
cataactgaaattaggaaaactcaccagggaaaactgggggtaaaatcaaagtataggggagcagtaggttcaagagcacccaaaactcgacgaggactc
aaacccaagaaatcaaatcaaaagattgcaatgttcaatgctgtcaaccccttgaaacttgccagttgccatagggtcttaataagcaggcaagcgacgg
cggcgacggatcttatgctcttgctcagtttgacctgcagcttctacgaccccttgctcagtttgatccacagctccaataactgcattctcaaattgct
ccacatcatcactgaccacagcctgaatcaggacggtggctagcaaagcgcacaggataatggctttcatcttgttccttcccagggggttggggatcgg
ggggggggggggggggggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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