XB-CLONE-4353194
Clone Name: IMAGE:7026072
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Gene:
atp5po
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783769 [+]
taattaatcccccccccccccgctcgctttgacctagtgcagcggccggaggagaatatggcagcgttagggagcttctcagtgaaggttcgttccttca
gcaccagtgctgtccggccaatttccaagctggtcaagccccctattcaggtgtatggcctggagggtcgctatgccactgcactgtattccgctgctac
aaagcagaagaagctggaccaggtggagaaggaactgaccagaatatcggcacttattaaggatcccaagttgtctggcgtcatcacaaacccacatgtt
aaacgcgccctcaaacagaagactgtcggcgatattatggcaaaggagaagctctcccctctgactgtcaactttgtcaacctgctcgctgagaacgggc
gcctgagtcaggcctctgatgtcatctcttcatttgcaaagatcatgagcgcgcaccggggggaagtcctgtgctccgtcactaccgcctcacccctgga
tgaagccaatctgacagaactgaaatctgctctcaacggcttcctggcaaagggggagacattaaagctggagacaaagactgatgcaacgatcctggga
gggatgattgtcagcgtgggagacaaatacattgacatgtccaccaaagccaagatccagaaactgaccaagatcatgagagagactgtgtaaccgatag
agactgctctgttctacccgctgtattcccggcagcgccacatcactataataataaattgccctgtgtccaaaaaaaaaaaaaaaaag
3' EST: CF783768 [+]tttttttttttttttggacacagggcaattatttattatagtgatgtggcgctgccgggatacagcgggtagaacagagcagtctctatcggttacacag
tctctctcatgatcttggtcagtttctggatcttggctttggtggactgtcaatgtatttgtctcccacgctgacnatcatccctcccanngatcgtgca
tcagtctttgtctccagctttaatgttcccccctttgccagaagcccgtgaaagcagatttcagttctgtccaaatggctttcatccaggggttgaggcg
gtattggccgaaccaaaggacttcccccccgggggggccccccctggatttttttgaaaatgaaaaagattaaacttttaaagggccggaattcaggggg
cccccggttttctttcgggggggggggttggtacaaaattttttcacccctccaagggggggggaaagctttttccccctttttgtggccaaaaaaaaac
ccccccaaaaaatttttttttttttttgttagggggggggggcctataaaaaaaaagagggggggtttttttttttgttataaccccccccccccaaaat
atttggggggtcgcctttcttaaaaaaaaggggggggcagaaatattattggggggggggggttttttttttcttcccccccccgcgggggggggcgggt
tcttttttttttttttttttttttttttctcgcccgccacaaaaaaaaaagggggggggggggggtggaacacacaaaacccccccccccccccgctgag
caagaaaaaaaaaga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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