XB-CLONE-4353189
Clone Name: IMAGE:7026069
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Gene:
atp5f1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783772 [+]
taattaatcccccccccccccccgagcctctgagtgctccttctgcactgctgctgctctccgctgctgacgccgccatgttaggggctgtaggacgttg
ttctgcgggggcactgcgggctctaaagccagcatctaacccagttcagctcgggcagaaccttctcagatgttcccctgcagcgctgcactcccggaga
gattatgcagctcagacttccgcagcagccaagcctggaacagcagtcggccgcattgtggcagtcattggtgctgttgttgatgtccagtttgatgaag
gcctcccacctatcctgaatgccctggaggtccagggcagagacaccaggcttgtgctggaagttgcacagcatctgggtgaaaacactgtccgaacaat
tgctatggatggcccagaaagtttggtcacagggcaaaaagtccttgatgctgggacaccaatcccaattcctgttggtcccggagaccccttggaaaga
aaccctgaaaggtaaattgggggaacccccatttgttgaaaagaaggggcctattttccaccccaaacaggtttttgcagccctttccatggcacaaaac
ccccaaaaaattttgttggaaaattaacccttttaacccggggaaatctttggggttaaccgggcctttaaaaagtttgtaaaaattttgcctggggccc
ccccctttggcccaaaaggggggagaaaaaaattggggcttttgttttggggtgggggaccgcgaagcccctgtttaaaaaattttcggccccacattat
tgtggacctttgtaataaaacaaaattg
3' EST: CF783771 [+]tttttttttttttttggtctttacatggatgttttattggaaactttaaatacactttgccaacataactcaagacagatacaggcattaagtgttactt
tgaggatgaaggtttcattgaagtgcatgaggaaaatagaaataagtctttaagagtgctcttctgcaagtttttcagcctttgttacagcttcttcaat
cgggcctaccatgtagaaagcctgttctggaagattatcatatccaccgtggaggatctgcttgaatcccttgatggtatcttttaatgggaccagttta
ccaagatgtccagtgaagacctcagctacttggaaaggttgggagaggaaacgctggatcttgcgagcacgggctacagtcagtttgtcttcctctgata
actcatccatacccaagatggcaatgatatcctgcagtgatttatagtcctggagtattttttgtacaccacgagcaacctcataatgttccagccccac
aatgttgggatccatgatacgagatgttgagtccagaggatccacagcaggataaatacccaactcagcaatagcacgagacaatactgtagtagcgtcc
aaatggggcaagggggtagcaagagctgggtcagtcaagtcatcagccagtacataaatggcctgcacagattgtaattgaccctttctttggaattgaa
attctcccctgcatgggaacccatggtcagtggccagaagttggctgggaacccaacggccgaaggggaatacttccccattcaagggcaaaaaaccttc
ttgaaaccacgccctgggggtaaaaaccttgaaaaaagggtttgtcccattaaaaaaaaagccaccggcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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