XB-CLONE-4353179
Clone Name: IMAGE:7026065
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Gene:
crip2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783778 [+]
taattaatccccccccccccagcaaggcagactgagggctccatcaaaagccaatggttaccaggaagtgactggaagccaggcttacatgtgattgtct
cagatgatgcttgcccttgtggctttcccaaaaagggctcttcatgccaattttgtggaacttttctgtgtcatttagatgcaatgaaagagtcaccttt
taatttaaacctaaagcacccagagtttttaggggcattgagaaaacagacaatgtatctctctgtctggatattggcagtgattagtcccataaagagg
attacttgttttgtttcttgaaacgggcaacccaccaactcttagcagcacaagcacacactatggtaatgggcaaggatgttaaatattgtggcacaaa
attgtatgtgcacttgccaaagagtatgcagagcatcaacatcatcatcatcgtcatcatcatcatcactgcttaccaaatgtaaggacctcagatacca
tacagtagcttcagcacaaattattccctgtgagtgggactcttattcatttacattgatgtctgacaaaattcttatatagaagatgagcaaccaccag
ctctattaatataatgggcattagttcaacttaaatatggcgtctcctggcagcttggcaggaattatgggtggctcacaaataaagagacagctaaagt
gatgccctacagccagaaccatttgtggtttgctaatttgtgcaactgggcctttttgttcaaaagttcttattaatttttaaaaataaaagaaccaac
3' EST: CF783777 [+]ttttttttttttttcctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttggggggggggggg
gggttataaaaaaaatccccgggaaaaaccaaaaaaagggatttcccgaaaattaaaatttgcccaaccccaaaaattttccttttttcagggaccaaaa
aaccggggtaaaatgcaaaaaaaaaaattccccaaccggaaaggcaaaaaaaactttggggggccaaaaagaaaaaaaccccccaaaaaaaaaaaaaacc
ggccaagggccccccggggggaaatttccccccccaccccccacccctggggggaaaaaaaaaagggaagggggcccccccccccccgaaaaacccaaaa
aggggaaacttttttttccaaaaaaatcccgggtcccgggttgggggcccttaaaaggggaaaaaaaaaaaaaacccccttttggggacggggggggccc
attttttcttgatttaaaccccggggggcccccaaatttaaaatagggcccccttcccgaaaaaggggggggggcccccccccaaaaattattttccccc
cccataaccccccaaaaaccccttaaaaatttgcttccttaaacagaatggcccccaaccccggaaggggggccttggattttaaaaccccaaaaaaagg
tcccccgggggcctttccccccccgggaaggaaaataaatccccggaaaaaggggagttgccccccattaacaaaaaaaaaacggggggtcccccccccc
ccgcccaaaaaaagggggagggcta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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