XB-CLONE-4353176
Clone Name: IMAGE:7026064
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Gene:
tuba5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783780 [+]
taattaatcccccccccccccgagttgctgtgttggcggagacccgtagcgcgtctatagtcaacggagccaaagaggggcgagtgtaggaagactgaag
gttatctggtcttttatttagttctaccggtgcgcacacatctaatcagaaaatgcgtgagtgtatatctgtccatgttggccaggctggagttcagatc
ggtaatgcatgctgggaactttactgcctagaacatgggatccaaccagatggtcagatgcctagtgacaaaactattgggggtggagatgattccttta
acactttctttagtgaaactggagcaggaaaacatgttcctagagcagtgtttgtagacctggaatctgcagtcatagatgaggttcgtaatgggacata
taggcagctttttcaccctgagcagcttataactggtaaagaggatgctgccaataattatgcacgtgggcactacactgtaggaaaagagatcattgac
ttggtgctggaaagagtacggaaactggctgaccaatgcacaggtctccagggttttctgattttccacagctttggaggaggaacaggatctggattta
catccttgcttatggagagactatctgtagattatggcaagaagtccaagttggaatttgccatctatccagccccacaagtgtctacagctgtggttga
accctataattccattcttaccacacacactacactgggagcactcctgactgttgcatttatggtgggaaaaagaaggccatttttggacatatgcagg
gggggaacctcgaacatttgagccgccccaaccctaaccttatttttgaaacgcccctccattggggcccaaataagttctta
3' EST: CF783779 [+]tttttttttttttttgaataaaaacctttatttcaagcagctatgtcaggaatgacattctgtataatgcacttcaatggaatggcactaatattcctcc
ccttcatcctctccctcaatagagtctgtgcccacttcctcataatctttttctaaagcagcaagatcctctcgtgcctcagaaaattcaccctcctcca
tgccttcaccaacataccaatgcacaaaagcacgttttgcatacataaggtcaaacttatggtccagacgtgcccaggcttcggcaatagctgttgtgtt
gctgagcatacagactgcgcgctgaactttggcaagatcaccacctggaaccgcagtaggtggctggtaatttattcccaccttaaaacctgttgggcac
cagtccacaaactgtatagaacgtttggtcttaatagccgcaattgcagcattcacatcctttggcacaacatctccacgatataacatacagcaggcca
tgtacttgccgtgtcgtgggtcacattttaccatctggttagatggttcaaaacaggcatttgtgatttcagacacagagagctgctcatggtatgcttt
ctcggcagaaattataggagagtacgtgacaagagggaagtgtatacgaggataaagcaccaaattggtcttggaattctgtcaagtccacattgagggc
cccatcaaagcgcaaggaggctgttgatataaaaagaataacctgggccaatgagggcggttcaaaataaatgttaggtctggggcccctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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