XB-CLONE-4353171
Clone Name: IMAGE:7026062
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Gene:
jpt1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077053
[+]
CCCGGAGACAGTTGCCGCGAATTGCAGTCATGACAACTACTTCCACGTTCAGCGGTCTGGACCCCGGTACTCGGAGCAGCTCCAGGGTCCTGATGCCTCC
AGGTGGAGGGTCAAACTTCTCTTTTGGGGTCGGCGAGTCTCAGGCTCAGCCTGTGAGGAAGCACAAGATGGCTTCTAACATATTTGGGGTTGTAGACGAT
GAACCAGCACCCACCAAACAAGCACAGGAGACAGGCACTCCTGATGCATGTGGTGAATTGGACTCTGCAGCAGAGTGGACATGTCAGTCAGAGGACTGCA
GCAATGCCAGTGGGAACATGGATGAAATGGCGATGCCAGAAACTGAATCTGGAAAGGAACAATCTCAAGTAGCTGGAGAACCCGCCCAGCCAGCACAGCC
TGCAGCAGCCCCAAGCAGAAGAAACCCTCCTGGGGGTAGATCCACTCTTGTTCTAGGTTGAATGTTATTATTGTGAACTGTTCCTTCCCTGGATTTGTCT
GTTCCTTCAATTTGACTCCACAGCTGACTGCACATATATAAGCTCCCTTGTTATTTAAAGCACTTTGGTCCTGCCATCTTTTCTACGGACGGTTCTCCAT
GTGTGTCCTGGGATCTACTGGAAGACGCATGAATTTCTAGTAGTACTGTTTCTTCTATTAATGTTTATCATAATAAAGAATTTGTCAATGGAAAAAAAAA
AAAAAAA
5' EST: CF783783
[+]taattaatccccccccccccccccggagacagttgccgcgaattgcagtcatgacaactacttccacgttcagcggtctggaccccggtactcggagcag
ctccagggtcctgatgcctccaggtggagggtcaaacttctcttttggggtcggcgagtctcaggctcagcctgtgaggaagcacaagatggcttctaac
atatttggggttgtagacgatgaaccagcacccaccaaacaagcacaggagacaggcactcctgatgcatgtggtgaattggactctgcagcagagtgga
catgtcagtcagaggactgcagcaatgccagtgggaacatggatgaaatggcgatgccagaaactgaatctggaaaggaacaatctcaagtagctggaga
acccgcccagccagcacagcctgcagcagccccaagcagaagaaaccctcctgggggtagatccactcttgttctaggttgaatgttattattgtgaact
gttccttccctggatttgtctgttccttcaatttgactccacagctgactgcacctatataaactcccttgttatttaaagcccttttggtcctgccctc
ttttcttagggacggttctccatgtgtgtccctggggatctactgggaaaacgcattgaatttctagtaatacgggtttcttctaataatgtttatccta
aaaaaaaaaattgtccattggaaaaaaaaaaaaaaaaggtttcccctagtttttaaaaccgcgccccaccccgggttggaaacttggcccctgggccccc
actttgttttatttggcaccttaaaattgtgttacaaatttaagccccattcgcccctccctattttccccataaaaaaagccatttttttttttccacc
acacctctccaaattgtgtgggggtggtggcccaaaaactcccatanaatggtgtttcatctattcctaagaggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: