XB-CLONE-4353147
Clone Name: IMAGE:7026053
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Gene:
odc1
,
h2ac17
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783798 [+]
taattaatccccccccccccctcttggtgacattgtgaaaaagcatgtacgttggtttaaagcactcccacgtgtcactccattttatgctgtaaaatgc
aatgacagcaaagccatcgtgaagactctctccctccttggtgcaggctttgactgtgccagtaagacggaaatccaactagtacaaagtattggagtgt
catctgagcgaattatctatgcaaatccatgtaaacaagtctcccagatcaaatatgcagctagctgtggtgttgagaagatgacttttgacagtgaagt
tgaactaatgaaagtggcaaggaatcacccaaatgcaaagcttgttctgcgcatagcaactgatgactcaaaagctgtctgccgcctcagtgtaaaattt
ggtgccacccttaaaacaagcaggctgcttctggagcgggcaaaagagcttaatgtggatataattggtgtaagtttccatgttggcagtggctgcactg
atcctcagacttatgtacaagctgtctcagatgcacgatgtgtctttgacatgggggctgagcttggattcaacatgcatttacttgacattggtggagg
attccctggttcagaggacgttaaactgaagtttgaagagattacatctggtgaaaacccagccctggataagtactttcccagaagattctggaatgga
ccatcctttggctgaaccctggggaaaaactatggggggcccctgggtttttacacctccgcagtttaattatcccttgcccaaaaaaaactttttggcg
ggaaatgaaccccaagtcgggaatcctgaatgaac
3' EST: CF783797 [+]tttttttttttttttgtttgaacactttatttgtaaaactggtcaatgaagatgctgactggcaatacacaagctacaaatgcttgactgataatttgca
tctccaatatacttcaaaaaacataaaaaaaagtttccctaggaacacagataatgtgaccccatctcacagtcatccattttgttgcattaatgccggc
caatacaatctataaaaaacgaatgagttaaatttcacataaatggccccaagactaaagttgcagttgaactacagcgttcgttaataatcctacactt
tgatgctggcagcaggacagatggtatgggcaagctcaatgccactctcctgggcacccgatacatagtgtgcgctcagttctggtaccctcaggagaat
gccatgtcctttaataccatgctcgagtttccagtgtgaccttcactttacatcgctacacgttgggtctccggaaatcccttgaaatgtttgatgtata
caaccaatctccttcatgccactctatgcttccccaaaatatacatacccttccttcccacccctggtcgcctctgggaccacgcccgaaaacccatttc
ttaggccccaccccatcccaaatttctaacaacaaaggcactgggcttccccctataaaataccccaggcccttaaggaaccaaatcaatacccctcctt
cccataatatgttgcgccccccacccgccaacatatatccacatacgcccctttataaaacaacctttgtgcgccctctctcccactccctcttgtgtcc
ccccctcttattcttcatctcccccaacaaacctctgatcctattattccacataaaaaagattttctctcactccaccccctaaatcctcaatcgacct
ctctatgacaaatatacgtgttaaccacactaccttcaggccacaacacagcccgctatcagctctggtgatctgatctcaccttctcccacactatatt
attttaaaacttaggggaaaagttctataaatatagagcctgcacaatttcccccctccaaatacgaaacggtctgtaattatgtgagttctcttattta
taccccccccctcaccattgtaaaacccgcccgccactttttacaagttcaaataccccaccgcccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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