XB-CLONE-4353104
Clone Name: IMAGE:7026035
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Gene:
nme3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077052 [+]
GGCTGGCTCTTCCCGCGGGCTCGCTCCCGCCCTTTCTCTCCGCTCTCGCCATGATCTGCTTGGTGCTCACCATCTTCGCCCACATCTTCCCTACAGCCTG
GACAGGGATTAATGAGCGCACTTTCCTGGCCATCAAGCCTGATGGGTACCAAAGGCGACTGATTGGCGAGATCATACGCCGCTTTGAGAAAAAGGGATTC
CATCTGGTGGCGATGAAGATAATGCAGGCCTCGGAGCAACTGCTCAAGCAGCATTACATTGCTCTGCAGGACAAGCCCTTCTATGACCGTCTAGTGAAGT
ACATGGGTTCCGGACCAGTGGTTGCTATGGTGTGGCAGGGCTTGGATGTGGTTAAGACTGCCCGTCTAATGATTGGGGAGACAAACCCAGCTCACTCTTT
GCCAGGCACAATCCGAGGGGATTTCTGTGTAGATGTCGGCAGGAACGTTATACACGGCAGTGACTCCAGGGAGAGCGCCCAGCGAGAGATCGCTCTTTGG
TTCCAACCCGATGAGCTTGTCTGCTGGCAGGACAGCACAGAGAGCTGGATATATGAATAAGGAGCCGCCCTACCATGTTCCAGTGCAGTCACTTCAGGCA
TTGAGGCTGTGCCCGGCTGGCGCTACAAGCTGTGTTTGGGAGCATCCCAGGAATTCCGTCCAAGAGAGAAGCATTAGTGGAGGATGCAGTTTCGCTCTAA
CAAATTGGCACTTTCACAGATTCTACATGCAGTAACCCCTTCTTTTAGAAAATTGATGTCACTTTTTATGCTTTAAAATAGAATTATTGGGAAGTAATGG
TGACTTTTTTACTTATAAGACTCACGTAGGTGCCAGTGAAGGGATGCAGCTGGCCGGGCTGTAACTGCCCATCAGGATACAGACTGTCACTTATTGTAAC
CACTCGTATGTCTCTAAGCTGCAGGGGCCTATGCAAATGCACTGTTATATGGGTTTGGCAATAGTCGGAATGGACTGGATGTGTGTTCTGATTCATATTC
ACAGGATATCATAACCATGTGTTAAGGGTATTTTGGGTAAAATCATGGTAAAGGCTACATGGGAGAGAATGGGCACAAGGTGGGAGCATCTTGGGGTCGA
GTTGTAGCCTGTATGGTGGTAATTTGTATAAATTAGGCATTGGAAATAACAAATTGTACATTAAAAAGCTAGTGCTATTCTGAATGTATAAGAAGGGGTT
AATGCCTAGGGGAATCTGGAAGCTTTTCTATCTTTTTAAAATAAACTAAAAAAAAGCCACAAAAAAAAAAAAAAAA
5' EST: CF783824 [+]taattaatcccccccccccccggctggctcttcccgcgggctcgctcccgccctttctctccgctctcgccatgatctgcttggtgctcaccatcttcgc
ccacatcttccctacagcctggacagggattaatgagcgcactttcctggccatcaagcctgatgggtaccaaaggcgactgattggcgagatcatacgc
cgctttgagaaaaagggattccatctggtggcgatgaagataatgcaggcctcggagcaactgctcaagcagcattacattgctctgcaggacaagccct
tctatgaccgtctagtgaagtacatgggttccggaccagtggttgctatggtgtggcagggcttggatgtggttaagactgcccgtctaatgattgggga
gacaaacccagctcactctttgccaggcacaatccgaggggatttctgtgtagatgtcggcaggaacgttatacacggcagtgactccagggagagcgcc
cagcgagagatcgctctttggttccaacccgatgagcttgtctgctggcaggacagcacagagagctggatatatgaataaagagcccgcctaccatgtt
ccagtgcagtcacttcaagcattgaggctgtgcccggctggcgctaacagctgtgtttgggagcatcccaggaattccgttcaaagaaagaaacattagt
gggaggatgcagtttcgcctctaacaaatttggcacttttccccagatttctacatggcagtaaaccccctttctttttaaaaaaattggaagggcccct
tttttttatagccttttaaaaataagaaatttattga
3' EST: CF783825 [+]tttttttttttttttgtggcttttttttagtttattttaaaaagatagaaaagcttccagattcccctaggcattaaccccttcttatacattcagaata
gcactagctttttaatgtacaatttgttatttccaatgcctaatttatacaaattaccaccatacaggctacaactcgaccccaagatgctcccaccttg
tgcccattctctcccatgtagcctttaccatgattttacccaaaatacccttaacacatggttatgatatcctgtgaatatgaatcagaacacacatcca
gtccattccgactattgccaaacccatataacagtgcatttgcataggcccctgcagcttagagacatacgagtggttacaataagtgacagtctgtatc
ctgatgggcagttacagcccggccagctgcatcccttcactggcacctacgtgagtcttataagtaaaaaagtcaccattacttcccaataattctattt
taaagcataaaaagtgacatcaattttctaaaagaaggggttactgcatgtagaatctgtgaaagtgccaatttgttagagcgaaactgcatcctccact
aatgcttctctcttggacggaaatcctgggatgctcccaaacacagcttgtagcgccagccgggcacagcctcaatgcctgaagtgactgcactggaaca
tggtagggcggctccttattcatatatccagctctctgtgctgtcctggcagcagacaagctcatcgggttggaacccaagagcgatctctcgctgggcg
ctctcccctggagtcactgccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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