XB-CLONE-4353082
Clone Name: IMAGE:7026027
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Gene:
tiparp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783838 [+]
taataatccccccccccccccactggacttgagctgctgtaatgggaagcttgtcacgtagggagcagcaggcgagagcgggctaggatccctccctcct
ccctctggaacagaggctcagccaggggacaaggaaccttactccagcctaaccccggaactggcacaacctccttgcaggcttgtctgaggcattttga
acccatggagaactctatacaaattatggatacagcgcctcatggggaatgttctgtgaaggtcccttgttctgacataagtggcaaagacatgagtcaa
agggacttttctatgcactccaacaaaattccaccactgaagccatgttttaaaaagaaacaggctccgaaattattagactcagaaacaataaatgctc
taaggccaataattactagttttttgggatctgggactctcgatgaaatatttgtgtctaggaatcgagcagggaatacgagcagtttatgtgagcccat
cgacaaaaaggcactggggatggagcagcagtgtcctccaactaattcactgatatctgggagtccaagtcttagccccttaacagaccaaccacctacc
gttcatgcagagcaggaagaccttgtggtacatcagcccactgtattagacacaacgggaaccaacattttccatcagacaatttcaaatatgactggtt
aataataatactggccatgggctcttttccaagattcctcttcctgcagaacaatctcaaacagattttcaccaattcctggcctgccttaaacaaaaa
3' EST: CF783839 [+]ttttttttttttttcctcattaaataaagattttattaacaaaggcacacaaactattaacattgaaaaatgtatttacaaaatgcacataattagcggc
aagtgcactgttggtcgttgtatgtccatcacgactagtagatttatttttcagtctctttctgttcccaccaatttaaataacagatcctaagagacat
taaagcagctttcatgttgttaaatggagacagtgttgctaacttcttcatactggataataaaataagggtagctctgatcatcgttaaatataacaaa
aatctggggctcaaagaagttgtccacacaagagtcatagaggtcactggtcaagctccctgggtttaacggaggaggccttctcatggtatgattccca
accacgtaccttccagttagcactttagccagaaacatatagtgaactcctttgggggagcgtttggaaaagttgtgtgaatagctggctttcctggcaa
agtaactgccctgtccaaacatggtggcatgcttcccgcacactctggggtcaaagttgtgtttgcaaattgcatcaacaacatcctgcgaggtcccgtg
gaacagatgcctttcattcataattctgtccagtcccgtcatcttccttgtcatgaattcttttttcctcttatatttttcccacaagaaagagattctg
gacgcgttattatcttttaaatgcggaaacctggttttctgggcaccgtttttgtggaaacaaagtaataaactggtccgggaacccttttgtcctccct
ttaaacataaggcactttgaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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