XB-CLONE-4353070
Clone Name: IMAGE:7026407
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Gene:
rpl4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783847
[+]
taattaatcccccccccccccccccgccttttctcttcgtggccgctgtggagaagcagcgaggagatggcggtcagcatggcctgcgcccgtccgctaa
tatcagtatactctgagaagggggaaacatctggcaaaaatgtcaccatgcctgcagtgttcaaggcacctattcggcctgatatagtcaactttgtcca
cacaaacctacgcaagaataaccgccagccatacgcagtgagcgagcttgctggtcaccaaacaagtgctgaatcatggggaactggtcgagctgttgcc
cgtattccccgtgtgcgtggaggaggaactcaccgttccggtcagggtgccttcagaaacatgtgtcgtggagggcgtatgtttgccccaactaaaacct
ggagacgctggcaccgtagagtcaacacaactaaaaagcgctatgcagtctgctctgcataggccgcctaagccctttctgctcttgcaaagtctaaagg
tcacccgtattgaggaaatacctcgaagttcccctttgttgttgaagataaagttgaaatgtatccgaaaaaacaagggaagcagttccttcctgctgaa
aaaagcctcgtatcctggtaacggacctcacaaaaagttttatgccccctttaacccttatgacgtgcacgaggaaaggggaaagaataaaggaacgaga
acgttcccatttcctaccgcattaaagaatccttagagttttgtttttacgatgggcaaattcaaatcggcaatgtggttacaacccccatttataaaaa
tcagcttccccctgtaagaccaatgccccgccataacgatagcctttgcgacaccgcctccaactcctctttttgacaggtttctgcgcgtccaccttgt
cgggggt
3' EST: CF783846
[+]tttttttttttttttcgtttgaaacaatttattgagtaaatccaaagtgctgaacaggaggaaacactccaggattactttgactctgctgctttagctt
ccgccctggcttgcctcttctttgctttggccaacttgatcattttagtttttgcatcaagtggcttctttgctttaggcttgccatcatctggcttctt
ttctttagctttaatattctcaagctgctgcaggatagcatgacgtcttgctgtctttgcatatgggttcagcctcatcatgattctcaggttcttcagt
gggttcttcttgagctcccttcacttcacctctttgtttggagcccgcaaagccctctggatctcctggcttttcaggattctggtcaaatctgtgtttg
tcatcttgtgcattggaaggttgtaatctgccttcagcctggctgatttgcgccacgtaccatagaggtcatccaatttgcggaaggcgctttctgtcca
aatgcagaaccgtccaacatgaccaccaggagcaagccttaaaaggttccacttgccttacattcaggaaggtgattcccggggaatcttttttaaaggc
cttttaccccggacccttatttttccactggtaaacaaacaaaaggggtcccccctgccccctaaaatgcaaaacgtgggtttcccccaatccattaacc
catttccaggcacggccttacccctggaaaacacaaaaaacccttccttttaaagaaaaataccaagggctctttgaagccttttttccactataaaaag
aacccggctctttcctttttgtttttacgcccaaacccctttcacacacctttatttcttttaaaanaaaaaaagaggggaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: