XB-CLONE-4353067
Clone Name: IMAGE:7026406
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783849
[+]
taattaatcccccccccccccgagagacatcttcgcagtcacagcagaaggaatccgcagttcttgcttcaacagtgcttgaacggaactcggcttctcc
aggctcgaatcgcaagtcgcatatccgcagtcttttttaacactttgccaagtccagcaaaatcgtgacatcatgagcgctcagagtcagatccgccaga
actaccatgaggaaagcgaggctggaatcaaccgcattgccaacctagagctgcagacttcctatgtctacctgtctctgggctattacttcgatcgtga
tgatgtggctctttccaagttctcaaagtactaccgtgagctatctgagaagaagcgggatcacgctgaggatttactaaaattccagaacaagcgtggg
gggcgcgttgtgctccaagacatcaagaaacctgatgccgacgagtggggcaatgggaccaaggctatggaggttgcactcaatcttgagaagagcgtga
accaggctctcttggacctgcacaaaattgctacagatcatgccgatccacatatgtgtgactatcgggagcgcgagttccttgaggaggaagttatatc
ctccagaagctgggcgaccaccttaccaacctgaaacgcgttaaggccacctgaaaatggcaggggaaaattatttctttgaaaaagcctgacccggggg
agacaaaggaactaaaggccctgtccccataaaatggtgttggggggggtttatattctgactgggccaggtccgccctcgccattccagggtttgtgaa
taattgggcgcattaaattgggaaatccaaggggttgaaaaaggggattctccgggaaaaagggaatatttcctaggtactaccacaataaaagagaaaa
aaatcaagtctttttcttaacggtgtttaagagaaaaatacaaataaa
3' EST: CF783848
[+]tttttttttttttttagctgctagtaaagttgtattttgcttatgtgcagatcaagcaaatcacattccagagatcacatttcaactttggattccaatt
aatgagcaatagtaagaagcgggaaggaggggcgactggccaggcagaatatgcaccccacacattttagtgacaggacttagtcactgtctcccagggt
cagcttatcaaagagatattctcccatgccatcttcagctgccttaacgcgcttcaggttggtaaggtggtcgcccagcttcttgatgatcttaacttcc
tcctcaaggaactcgcgctccagatagtcacacatatgtggatcggcatgatctgtagcaattttgtgcaggtccaagagagcctggttcacgctcttct
caagattgagtgcaacctccatagccttggtcccattgccccactcgtcggcatcaggtttcttgatgtcttggagcacaacgcgccccccacgcttgtt
ctggaattttagtaaatcctcagcgtgatcccgcttcttctcagatagctcacggtagtactttgagaacttggaaagagccacatcatcacgatcgaag
taatagcccagagacaggtagacctaggaagtcctgaactcttaggttggcaatgccgttgaattccagccccgcttttcctcaagggtagttactggcg
ggatccgaactccggaaacgcctcatggaaggtccacgaattctaggcttgggaacttgggccaaaacgtggcttacaaaaaaaaaaactggcgcgggag
atattggcgcacatttttgtcggaaatattccggaggtctctttggggaaaaaaacgccttcaaaaantttcccccccgctttcttaaaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: