XB-CLONE-4353059
Clone Name: IMAGE:7026403
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Gene:
sec61b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783854 [+]
taattaatccccccccccccccgagtaagaccagttgtagtacagagttgctgtaaaggtgcacatagatcacaatgcctggaccagcagcaagcgctac
caatgtcggtgcatcttcgcgatcaccaagtaaagctgttgcacccagaactgcaggttctaccgtcagacaaagaaaaaatgcaagcagcagcacaaga
agtgcaggtcgtaccacatcagcaggcacaggaggcatgtggcgattttacactgaggattcaccgggtcttaaagtcgggccagttccagtgctggtga
tgagtctactttttattgcatctgtctttatgctgcatatttggggcaaatacacacggtcttagattctatcctctccgctctgaaatactaccatcag
gatttggaccaaatgttttatctgaattattctgcagagaataaggatataaaaaaaaaaaactgcattataaattagtctgcctaatgtgaaacgaatc
caaagtcaagacaaaagtgtgctttgttttgctactctgctcattaaccattggctgcggcttcccccgataaacaggagcactattgtccctcctgacg
attacaaccactgtatctgtcaaaatacgtatccttacaaccaaacacaaaatgcggttcttttccgaactacaaatccccctcaaaaaaagttagtaaa
aacatccctggttcccatccatttattgtccacccctattttaaataacacctatttccccttcctcaatcccttaaatacaaatgcccccttatacaga
tttctattacacccaccacccccctttcttcgtttccacccctgtgacacccgcccacacacacatagtcccct
3' EST: CF783853 [+]tttttttttttttttctttttttttttttttttttttttttttttttaaacagcatagtttattgaaaggttgacaaaatgaatgaacaagaagtcttac
aacctgttaatggagatatgaagtcagaaagaaagcacttgtgttagttctaaactatgtatcttctcagtacattggttctgatcctcttgagtgacaa
tactgctcctgtctatcgtgcgaagcagcagccaatggttaatgagcagagtagcaaaacaaagcacacttttctcttgacttttgattcctttcacatt
atgcagactaacttataatgcagttttttttttttatatccttattctctgcaaaataattcaaataaaacatttggtccaaatcctgatggtagtattt
cagagcggagaggatagaatctaagaccgtgtgtatttgccccaaatatgcagcataaagacagatgcaataaaaagtagactcatcaccagcactggaa
ctggcccgactttaagacccggtgaatcctcagtgtaaaatcgccacatgcctcctgtgcctgctgatgtggtacgacctgcacttcttgtgctgctgct
tgcattttttctttgtctgacggtagaacctgcagttctgggtgcaacagctttacttggtgatcgcgaagatgcaccgacattggtagcgcttgctgct
ggtccangcattgtgatctatgtgcacctttacagcaactctgtactacaactggtcttactcgggggggggggggggggaataatttaacttgagtgcc
aaaaagaaaaagtaacttggtattctcgggcccggaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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