XB-CLONE-4353040
Clone Name: IMAGE:7026394
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Gene:
luc7l2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783865 [+]
taattaatcccccccccccccgatggtgtaacgctcacgcaagcgagagcagaacgctgaagctgttgtcctgtctgtcgccggccgctgtgtgaggagc
gagtcggctgctattttttattttcttcttcccttcgtcgaagcaccgtgccgttgtgtgaatcgtttacgtcgccaaggcagttcctacacgtctagaa
ctccccccccctcccttatcaaaatgtcggcgcaggcccaaatgcgcgccttgctcgaccagctcatggggacctctagggatggtgattctacgcggca
gcgaatcaagttcaatgacgaaagagtatgcaaaagccacctgctcaactgctgtcctcatgacatcctctcaggaacgaggatggatctgggagaatgt
cttaaagttcacgatctggccttaagagcggattatgaaattgcatccaagcagcaggactttttctttgagcttgatgctatggatcatttacagtcat
tcattgcagattgtgacagaaggacagagattgccaagaagagactggcagatacgcaagaggaaataagtgcagaagttgctgctaaggcagagcgagt
gcacgaactcaatgaagaaattgggaaactgctggccaaagctgaacaactgggagctgaaggaaatgtggaagagtcacaaaagtcatggatgaagttg
aaaagaccagagtcagggaaaagaaaagctggaggaaatctaccggtaatttccttgcctggcatccggtttttcaacaaacaaaagactacctggttct
gtgaaagttctgcttctgcccttacttgggggtccttccttggaccaatggaaccgaaaaaaattggggctggatacacctttttggggggggaaaaagt
ttcccttttgtggggggattttctttttggaaat
3' EST: CF783864 [+]tttttttttttttttcacacacaaaatgtctttattttcataagagcctttacagagaattttggaaaaatggcacaattcaaaaacaaaacaacatttg
tcgttaatgtagttgtgattttgcttaacaaaacaaatgaaaagttctcaatattcatacagatatattataaccagactagaccactgctcaatttccc
ccattaataagctaaatacaaagtagaaatatcaatccctggattttgtttatccaattacttatccatccttttttccgaaatactactttggctgaca
gagccattatgctaactgctaccttaccgtattactttgcaacttattaaaaagttccagcaactgatagtaatgaatgtcgatctaaagaatcaaaagt
aaagctgtcatggtatgaagcaaaagcatggtaagcatacaaagcagacctgcaatctcggtaaaacagccactttatattcagttggttctcagtttaa
agaaactcagagctgaattagaacgcagtagctaatgttctcccatccaattccacagaaggaagtgaagatactgcaaggcacagaagtaacttcagca
gctcatgcacctggactacacaattttaatttgtatctaacaaggggaaaaaaaagcattaagctaataaattgtttggctgccaccataagagacaact
gttgtgattaagttcaactttaattggattaaattgtttattatctggacctagcattagattttagatcactgaaactgcttcaatgaaaaagcagatg
gtaaaattcagctttctaggcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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