XB-CLONE-4353031
Clone Name: IMAGE:7026391
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Gene:
XB5814120
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783871
[+]
taattaatcccccccccccccggtgaccttctggatccaccatgctgctgtacctgtgcctgttcctgctgggtgcctccactgccaaagggcacacatg
tacagacgttcctggcaagctgcagcagttggacgctggtgctggtgaggtttatggtatcacaaccagtggaagtatttacaagtggcaaaggaacatg
tgggcccagcttcccggagaaatgactcactgcgagtgtggggccagccggagtatgggcaaccaacaaaggcaatttggcttctaaacttcccgataac
acctggatgatactatcacgactccctgaatcaactaaatgctcggggtcacaaatatgttgcctggactgaacccacctagagtttgtttcacctgcca
cacgaccctattgacccccccttcccagatcagtaaaactgcatatgctcaccctaaaataccgaatgggataaccacgtagtatcgaaaattccggctg
gcggggaaattgcggaaatacaaaagggacggtaaaaaaaattgtgttacaacaaagatcctattttacctgtttataaggcgctatacacaatatttcc
atcaatatccctgaacaaattgcacaccccaaactataatctttaagaggcaaccttaacagaaaaaactccctcgattaatcccttttatataaggcct
aactaaaacaacaaaagcactattatacccctcttttatactcagaatcacccacagtgcaagatcttttaccccgctcgccacgctgttatccacccgc
tccctcccgcagatcaacaaaaaataatggctttacgacatatgatctcctcttataacacaccgcttatcactctatacataaacgccttctctcactt
tttttagtaattcacctttgctttctcaatataaagttaactccttttctcataataaatcttccattcctctatatttttcccatttaccattacccaa
aaaaactccacaatccccccgtttatactcttataaattatcacccacccaccccacattcgtcttttcacccgcttttctccgcgcactgccgcaatac
ccc
3' EST: CF783870
[+]tctgttccaacctccagcataacaagactgccttccaccctccaccattggctgccatcacaagccgtgggtttgacgccatagcggaagtagacttgtg
aaacattgtttacaccccagcatcccaagcccccacagctgtaatacacaagtctccctggtattggggtgaactgtacatatggtgatctggaaatggt
gtcatactgcttgaggcagtaaacaactcctgttgggttcactccagccacatatttcttacccccagcatctacttgcttcaggagtcctgatactctc
atccagttgttatcctgaagtttgaaaaccaaattgcctttgttggttgcccatactccagctggccccacactcacgtgagtcatttctccgggaagct
gggcccacatgttcctttgccacttgtaaatacttccactcgttgtgataccataaacctcaccagcaccagcgtccaactgccgcagcttgtcaggaac
gtctgtacatgtgtgccctttggcagtggaggtacccagcatgaacaggcgcgggtacagcatcatggtggatccagaatgtcaccgggggggggggggg
attaattaatttcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: