XB-CLONE-4353025
Clone Name: IMAGE:7026389
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Gene:
ston1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783875 [+]
taattaatcccccccccccccccgctcattgcaagctttacaagctcggagacggcgagcagctcatttaaaaccagtcgtccatatcacattactgaga
gcaacaatgtctaagggaccagcagttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgccaatg
accaaggaaacaggaccacacctagttatgtggcttttacagatactgagagattgattggagatgcagctaaaaatcaagttgccatgaaccccacaaa
cacagtttttgatgccaagagactcattggccgcagatttgaagatgcagttgttcagtctgacatgaagcactggcctttcaatgttattagtgacagt
ggaagaccaaaggtccaggtagaatataaagcagagaccaaatctttctatccagaggaaatatcatctatggtcttaattaacatgaaggaaatagctg
aagcctaccttggcaagactgtaacacatgccgtaattactgtcccaccaaattttaatgactccccagcgaacgggttaaaaagaagccgggtactttc
acccggggccgaatgttcctggttaaacacttacatgagcccatcggccgcctgtcaccggcctaagggaacactgaaaaaaagggttgtaaccccaaaa
gaaaagcctggctggaaaccttttgaatctctcggaggcagggcccaccccagggtgggccccccccacaacaaaactatgcttcgagggagttgcccac
tcatctttaccatttaaagggcttcccccctcttgcgggtttaccattcctcaactctccgcggtgggtatttacacacatatttccgtcgatataactc
ttaactacc
3' EST: CF783874 [+]tttttttttttttttgagagccaattcaattcattttattggtctggacccaatttgtttgtacagtacagatactatggttttcctgacttttggagtt
aattcaagtattaatgccccaaaattgacatacagcagcatacatgagaaaatttgaacagctagtacgaaatttgtgtcctaggagttacaagacaaaa
aagtggaatgcagcttttatttagtcaacctcttcaatagtaggaccagaggatgctcctcctgatggggcaccaccagcacctgggaagccaccaggca
taccacctggcatgcccccagcactttgatatagctttgtaataatagggttgcacaccttctccagctctttttgttgatgttcaaactcttctttttc
tgcagtctgatttttgtccagccaagatataacttcattgcatttatccaggattttctgtttgtcttcatcactaatttttcctttaagtttctcatct
tcaacagtagccttcatgttaaatgcgtaagactccagggaattctttgaagataccttatccctttgcttctcatcttcagctttgtacttctcagctt
cttgaaccatacgctcaatatcttccttgctgagacggcctttgtcattggtaattgtngattttggtcctcttttcctgtgctcttgtcaacagcagac
acatttaagaaacccattggcatcaatgtcaaatgtcaccctccatctgaggaaaaacctccgggaagctgggaaggaatgccaaatccacctcaaattt
taccccaaccaagttggttaacccttgggtcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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