XB-CLONE-4353022
Clone Name: IMAGE:7026388
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Gene:
gcsh
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082740 [+]
AGGAAAATGGCGATGCGTGTGCTGAGCGCGATGAGAGTCCTGAGTACAGTGACCCTTGTGCGGCGTCCCGCCCAGGAGTGCCGCCTGGTATGGAGGTCGT
GCTTGGTCAGGAGCTTGGCCACCGGCTCCCAGTTGCTCTCCTCTGCCTTGAAATTTACAGATAAACATGAATGGATATCAGTTGAAAATGGAATTGGAAC
AGTAGGAATCAGCAAGTTTGCCCAGGATTCTTTAGGCGAAGTGGTGTATTGCAGTCTTCCAGAAGTTGGCAAAAAGCTGAATAAAATGGATGAGTTTGGG
ACTTTAGAGAGCGTTAAAGCTGTAAGTGAACTTTACTCCCCCCTAACAGGAGAAGTTACTGAGATAAATGGAGCACTAACAGACAATCCAGGCTTGGTCA
ATCAATCTTGTTACGAAGAAGGTTGGCTAATAAAGCTGACAGTAGACAACCCTTCAGAGCTTGATGATCTAATGAGTGAAGACGCCTACGAGAAATACGT
AAAGGCACTTGAAGACTGATCAGATAATTTTTTGGAGTTGTAACCCAGCTAGAAGGTTTTCCCTGTGTAGGTTGAGTAGTGGTGTCTCAGATCACACTGG
TGGGGACTTCTTACTGCAACAGTAACGGTTTTAATCAAATGCAGTTTGCATAAGGACTGGAATAAGGGATTCTTTTTCAAGAGTTGTCCCTAAATCTTTT
GCACACTATGTAATTCCGCATTTTTTTATTTTTAGATATGAATAATTATGCTAAAACTTAGTAAACCTTAATTAACATGCTGCAATAAGTTCAGATTCTT
GTGTAATTGCTGCAGAGTTCTCCAGCAACTGAAGAGTTTATTGAACATCCGGTAATGTAGTGATTTCTTTAAAGACTTCTTCCTTGTGTGCTGGAATGAA
AACCATCCCTTTTTTGGTACCAACAGCCATGACTGAAGACAAAGTTTGTTGCACGTTCAGAAATAATGCCTTGTAATTAAAACTGATATGAGACCTTTAA
AAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF783877 [+]taattaatcccccccccccccaggaaaatggcgatgcgtgtgctgagcgcgatgagagtcctgagtacagtgacccttgtgcggcgtcccgcccaggagt
gccgcctggtatggaggtcgtgcttggtcaggagcttggccaccggctcccagttgctctcctctgccttgaaatttacagataaacatgaatggatatc
agttgaaaatggaattggaacagtaggaatcagcaagtttgcccaggattctttaggcgaagtggtgtattgcagtcttccagaagttggcaaaaagctg
aataaaatggatgagtttgggactttagagagcgttaaagctgtaagtgaactttactcccccctaacaggagaagttactgagataaatggagcactaa
cagacaatccaggcttggtcaatcaatcttgttacgaagaaggttggctaataaagctgacagtagacaacccttcagagcttgatgatctaatgagtga
agacgcctacgagaaatacgtaaaggcacttgaagactgatcagataattttttggagttgtaacccagctagaaggttttcccctgtgtagggttgaag
tagtgggtgttctcaagatcacacctgggtggggggactttctttactggcaaacagtttaaacggtttttttaaatccaaattgccagtttttggccca
taaaggggaccgtgggaaaataa
3' EST: CF783876 [+]tttttttttttttttctttttttttttttttttttttttttaaagggcccatatcagttttaattacaaggcattatttctgaacgtgcaacaaactttg
tcttcagtcatggctgttggtaccaaaaaagggatggttttcattccagcacacaaggaagaagtctttaaagaaatcactacattaccggatgttcaat
aaactcttcagttgctggagaactctgcagcaattacacaagaatctgaacttattgcagcatgttaattaaggtttactaagttttagcataattattc
atatctaaaaataaaaaaatgcggaattacatagtgtgcaaaagatttagggacaactcttgaaaaagaatcccttattccagtccttatgcaaactgca
tttgattaaaaccgttactgttgcagtaagaagtccccaccagtgtgatctgagacaccactactcaacctacacagggaaaaccttctagctgggttac
aactccaaaaaattatctgatcagtcttcaagtgcctttacgtatttctcgtaggcgtcttcactcattagatcatcaagctctgaagggttgtctactg
tcagctttattaaccaaccttcttcgtaacaagattgattgaccaagcctggattgtctgttagtgctccatttatctcagtaacttctcctgttagggg
ggggagtaaaattcacnttacagcttttaaacgctcttctaaagtcccaaaactcccatccattttatttccgccttttttgcccaaccttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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