XB-CLONE-4353001
Clone Name: IMAGE:7026380
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Gene:
capza1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783891 [+]
taattaatcccccccccccccccagtcggagccaagatggctgattacgaagatcgggtgtctgatgaagagaaggtgcgcattgcagctaagttcatta
cccatgcacccccaggagaattcaacgaggtgtttaatgatgtcagacttctactcaacaatgacaacctgctcagggaaggagcagcccatgcttttgc
ccaatataacatggatcagtttacacctgctaagattgaaggctacgatgactcagtccttataacagagcatggagatcttggcaatggcagatttgtg
gatccacggaatcggatttccttcaagtttgatcacttaaggaaagaagctagtgatcctcatcctgatgacagtgatgcatctttaaaatcctggagag
atgcttgtgatgtggccttgagggcttatgtaaaagaacactatccgaatggtgtctgcactgtttatggaaagacaatagacggacaacagacaataat
atcttgtattgaaagccaccagtttcaacccaagaacttttggaatggtcgctggagatctgaatgggaatttactgtgttcacaatccactggctcatt
tgggcaggagtttccaaagaattcagggttcattcccaattgaggcatgggaaaaccgccccagtttaagtttaaccccccacacagaatgtttttaagg
gagatcccctttaggacaattcccccctgggggggaaaagactttcaacaaaccctgggccattagtgggt
3' EST: CF783890 [+]tttttttttttttttggtgcaaatatttttatttaggatttatacacattttccccaatgatcatatacctgtggtacaaactgtaggtgccacaaaccc
gttaaagcgctataagacatttgcatttctgcatttttttttttttacatacacaagccattacccccaaacgatcatttctcactctctagaataaaaa
aatctatagcgtcataaaatgatgttcacctcaaagtaaggctagtaccaagaatcagctcctacaccggcaccagccttctaccctgcctgcaccccga
agcactgtctccaccctgagtgcaggcacaagcagcagatatgggctgtgaaatgtgaactctgtgcaatctgtttgccgatatccaccacgtctgcctg
cacccaggcagagacaacgcttccgggtgcagacaaggcagaagggctggtcccagtgtttttccgcaggctgagaatcagccctgtgtggcactagccc
aagaagacaaaagccaaagagaaaaaaacattttaaggtaaggagacagaatttcaagttttgtctgttgggttaccaaaacacaggttcccccaatgtt
gggtactgaagtaacagattcttagcattaattgggggtattttcccttatgtacactggaaacaggcatggtaacatctctttccagttgaattaaatc
ccaggttttgcagctttttttctttaaactgcattttcaaaaatcactttttgcccaaagcaaaaaaaaaaaccccccccaaacaaaaaaaaaggtggga
cccgtaaaaaaaaaatgggacta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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