XB-CLONE-4352998
Clone Name: IMAGE:7026379
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783892
[+]
taattaatcccccccccccccaataaatacaaatgttggttcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaggggtcccctttttttaaaggggccccccccccgggggaatttggccggggccccatttt
ttttttgcccctttaaagggttacaaaaaaagcaataccctcccaaatttcccaaaaaaaacatttttttccccgccttccaattggggtttgcccaaac
cccccaagggttttttaccggggggggtggggaaataatttgggcccccccccgggcctgaaaaaacccccaaaaaaaaaaattggttaggtcccttttg
aggggaaaaaaaccccctggggaaaggggttccattaggggggggaaaatccccaggcccccccccggggaaaaatttgcaaaacctgcctttcattttt
tccccaacccgggggggaaaatccccaggcccccccccggggaaaaattttcaaaacctgctttccattttttccccaaccatattcccccccctaaatc
cccccatccccccccaaaatccccccagtttccccctttttcccccccaggggggaaaaattttttttttttattcaaaggcccgaagcccccctcgcgc
tttgagttttttcaaaaaattgtgggggagctttttttggaaagaataaaaccccccagggcctttttcaaaaaaaacttgtttaaaaacctttgggcac
atgggccccgtctgttttttacaaacgggtccgggacg
3' EST: CF783893
[+]tttttttttttttttcttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttcc
acccccctttttttttttttgggggggggggggattattttcccccggggcaaaaaaaaaaaatttttttttttccccccccaaaaaaaccaaaggccat
tttcccccgggagggaccccccttgaaaaaaaaagaaaaaggaatttttttttttttgtccccccaaacccatttttttaaaacacaaaggttttttttt
ttttttacccccttcctaaaaaaccccagggggggggccctaaacccccaaagggggatttttctcccccctttcccggggaaaccccctttccatttcc
ccctcccccccccccaaaccgaaattcccccccgtttttcttttggggggaaaacacccctggagggggccccccccccttctcagtttttctaaaaaaa
aacccccctttttataacccccccccccgaacccccccccccccctttttcctctttgggggggaaatttttacaaattttttaaaaaaccccccaataa
ttttggtccacaaaaaaaccccccccttttcaccctaatggggggagaaaaacattgaaaaatccccccccgaaatataaaaaccccttattctcccgcc
cctcttttttttttttctcccaaaaacccccccccccctcctggggaataccccccgatatccaacaaatcccgtgattttttccccccccaatgataga
aagaacacccccaccaaaggcccccttttcccgccgccaaattaatccctgcgtgggcgcgccctttttttgcccacatctttttcaacctccacct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: