XB-CLONE-4352990
Clone Name: IMAGE:7026376
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Gene:
ppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783898
[+]
taattaatcccccccccccccagaggtgagtcgtcttcaagcccgtctttctgaaatagaattcaacaactccaaggccaacaaagaacttgaatacttg
agagatgaggtccataaactacagatggaaaagcaaaatctgcagctggagacccgccacctccactccgaaatacagatcaccatgaaggaagctcaag
atatccgaaacatgactcaagtcgacagtggagcgaacttggattctcgcctcctctgcctggaagaagagctgaaagagctccaaatgatctccaaaga
caaagatgcagagattgagcaactgcaaaagcgtctggaaaccatgacaatcaagaaggaacagagagagaaccaccttcgccgttccatcgtcgtcatc
gacccagacaccggaagagagatgtcgccggaggaagcccgcagaacaggtctgatcgactggagtatgtttgtcaaacttcagaaccaacagtgcgact
gggaagaaaccaccatcaaggggcccaagggcgaatcctccgtgattatcgataggaaatcaggccggaagttcgacatcaaagaggccctgaaactgcg
gcgcctcaccagaccgccggtacgacagttactctcaacaaaggaaatggcccatccagaaattttgcagcccttggtggtctggcgaaaaacggcacgg
agaaaaacccactgttttgccatttatatttaccccccttttaagggggaaatgttacgggaccatttgaaatcccctttttacctgcccccccggggtt
tttcctgggtaaacccacaaaacaggggaacttccctattcccaccgtttgttttttcaccttttcaaaattcttttttt
3' EST: CF783897
[+]tttttttttttttttcagaaccaccacatctttattagcattacataaataaaatacatttgactatttctccgatcccaccatggaataagatatacag
aaagaggggtgccatggcaccatgatatcacacagggacaggtttgggttagtaaggcacatcattggctggaaaaagtgacagaagacagagctgtgct
tttctaaagcatccgctatcctttctctatatcacaagggggtgataagccccaatacaatctgtgggttttgcatctggtttatacagtcgaagtgatt
atcctgcttagtgcatgttccataatgagctttggcttcatatctacaccaagaatatactggaactatggggtacacttatggccactcccccctcgct
ggctgttaaagggatgccacaggacacgggcagaaggttgtcagaacccatgtccgtgtgaaaatatatcgtttctacaaactataaaaggagttaaagt
agaagaaacgtggaaaggaagtcccgctacgggtaacaggaaaacacggggcagttgtactgattcaatgtccgtaacatccccttaatgggcgtaatat
aatgcaactgattgttttctcctacagtctcccagacaccaaggctgcaaattcctggatggacatttccttgttgaggtaactgtcgtactggcgtctg
gtgatgcgccgcagtctcagggcctcttcaatgtcgaacttccctgctggattcctatcgaaaatcacgggaggatcccccgttgggccccct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: