XB-CLONE-4352987
Clone Name: IMAGE:7026375
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Gene:
ctsv
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783899
[+]
taattaatcccccccccccccgctgcaatttccagcaaaacagcctgagaggatacatcatggcactctatctaggtattgctgctatctgcttaaccac
agtgtttgctgctccaacaacagatccagctctggataatcattggaatctctggaagaactggcataaaaagtcctatgcaccaaaagaagaaggatgg
aggcgagtgttatgggagaaaaacttaagaatgattgaattccacaaccttgaacactctctaggaaaacattctcatagcctgggaatgaatcagtttg
gtgacatgaccaatgaagaatttagacagctgatgaatggctacaaaaaccaaaagaagataagaggttcaactttccttgctcccaataactttgaatc
acccaagtctgtggactggcgcaaaaaaggctatgtaacaccagttaaagaccagggacaatgtgggtcatgctgggcattcagtacaactggtgcgctt
gaaggtcagcactacaggaatactggtaaaatgatatctctgagtgagcaaaatcttgttgactgctccagagcccaaggaaaccagggatgcaatggtg
gcttaatggatcaagcctttcagtatgttaaggataatggaggcatagattctgaagactcctacccatacacggctaaggatgaccaggaatgtcacta
tgatccgaactacaattcagccaatgacactggtttttgttgacgttacatctggaaagtgagaaagatctcatggaatgcagtaagcttcagtggggac
cagtctctg
3' EST: CF783900
[+]tttttttttttttttgtttttttttttttttttttttttttttttttttttttttaaaattgaattaacaagtttttattttacattataaaagcttaaa
tcacataaaacacccattaaaatcaacatgtaaacaatactaaaacaacccagcttcaatgggggatgttaacttccaacacaaatttaaaaaacagcct
tgagcagcaattccatcagggaaaaacagtccagtacttgataaaaggggagtttcacaatatgggcacataaagttccaagggcaaaaagggccatatg
caatggtttattttaaaccaaaggataactggcagctgttgctattccacaggggttgggtctatccttggcaatgtatatgtatccatcgttgccccat
ttctcgctccagctgttctttacaatccagtatttcttgccatcctcatcttcaccctcaaatccatagcctacaacgagtacaccatgatccaggtctt
cactgctgcattcaggctcataataaatcccagatttataaaactgaaatgactgatgtcctgcatcaactgcaaaagagactggtcccactgaagctac
tgcattcatgagatctttctcacttccagaagtaacgtcaacaaaacccgtgtcattggctgaattggagttcggatcaaagggacaattcctggggcca
ccttaacccgtgtatggggtagggagtctttcaaaaatctatggccccccattatccccttaacataactggaaagggcgtttgggtcccatttaaaggc
cccccttttgcgatcccctgggggtttcccttgtggggcctccggggaagccaatcccaacacaaggaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: