XB-CLONE-4352973
Clone Name: IMAGE:7026368
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Gene:
emp3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084511
[+]
TGAGAGCCAAGGGGGATAGCAGAGGGGCTGCTGGCCACTAATATTAGCCATCCAGGGGCTGCTAGTGTATGCTGGGCTTAACTCCAAGAACTGAAGATGA
GCTTCCTACTCTTTGGCGTCACTGCCTTACATCTCATCATACTGGTGATCCTCTTCGTTGCCACACTTGACAATTCCTGGTGGGTATTCTCTGAAGAGGA
GACTGTGAACCTCTGGTACGACTGCCTTCTAGACAACAACACGGGAGTCTGGTTGTGTTCTTCTGTATCTACTAATGAATGGTTCCATGCGGTCCAGGCG
CTCATGGTACTGGCAGTCTTGTTCTCCAGCTTTTCCTTCATGCTGTTCATGTGCCAGCTCTACACCATGGAACGCGGAGGACTATTTTATGCCACTGGTG
TTTTCCAGATCCTTGCAAGTCTTGCTGTATTCTCTGGAGCTGTCATCTATGCAACCCATGTAACAGAATTCCACCCAGAAAGGGCAAAATCAGGTTATTT
TGGCCACTGTTTTGTATTAGCCTGGGTGGCCTTCCCCCTGAGCATACTGAGCGGAATCATGTACATCCACCTCCGGAAACGGGAATAAGGCAGTCTGTAT
TCATTTTCTCCTTCCAAAAATCAGGGACAAGCAAGAGGATACCAGAAAGATTTGAGAACCTCAGTGCCAGCCCTGGAAATGGACACCATGCTCTTAACCA
ACACTATCATTTTCATCCACATCACAAGTTCACGTAGCGGCCTTAACTCTGGCTAGAATCTTGTTGGCTGAGCCACAGCTCAGTGAGTACAACAGAGAGA
TTAATTCGGAAATGACTTTAGAATGTGTCATTCGATATAACAGTCTGTTTGCATAGCAACCGTCCCACACAATTTATAAAATACAGACGAGCCGCTAAAC
CACTTCACTTTTACATTAAGAACCAACATGAGTCATTGGGATTCCCCTTGACAGTTTCCTTTCCAGAGCAAAGCTTTTTGTGCTGTCACTTCTGCCGTAG
ATATGTTGCTCTTGTATTGAGGACAATGACGGCAATGGGTTTTCCAGCAGAGGCACTTACATCTCTGATGGAACTGTCCTATCAGCAATAATCTTCATTC
ACCACCGCTGATATCTCCATTATATTCATATCTCTTACGATGAACCTCCTACGTGGCTCTTTGGATTAGATTGGACGAACTTTTTACTTTTATAGTCAAT
GCTGATTGTTCTCTGCTGTTTTCATTGCCAATTACCACTTCTGGACAATGCTTCTCAATGTCGTCGCTGGTCAGAAAGTCACCAGTGTGGCATTGCCCGT
CTTGTGGGTATATTCTGTTATTGGAGGGAATTCAAATTGCCTTTATCAGTCACCAATGACTCCTAAGTAACCACATCAAGAGTATATTGCAATAAAGTTC
TGCCAATTCCAATAAAGGACCAGTGAGAATTGAAAAAAAAAAAAAAAAA
5' EST: CF783909
[+]taattaatccccccccccccctgagagccaagggggatagcagaggggctgctggccactaatattagccatccaggggctgctagtgtatgctgggctt
aactccaagaactgaagatgagcttcctactctttggcgtcactgccttacatctcatcatactggtgatcctcttcgttgccacacttgacaattcctg
gtgggtattctctgaagaggagactgtgaacctctggtacgactgccttctagacaacaacacgggagtctggttgtgttcttctgtatctactaatgaa
tggttccatgcggtccaggcgctcatggtactggcagtcttgttctccagcttttccttcatgctgttcatgtgccagctctacaccatggaacgcggag
gactattttatgccactggtgttttccagatccttgcaagtcttgctgtattctctggagctgtcatctatgcaacccatgtaacagaattccacccaga
aagggcaaaatcaggttattttggccactgttttgtattagcctggggtggccttccccctgagcatactgagccggaaaccattgtacattccaccttc
ccggaaaacggggaaataaaggccaagtcctggtaattccaattttttcctcccctttcccaaaaaaaattcaagggggaaacaaagcgcccaaaagaag
ggaaaatacccccccgaaaaaat
3' EST: CF783908
[+]ttttttttttttttttcaattctcactggtcctttattggaattggcagaactttattgcaatatactcttgatgtggttacttaggagtcattggtgac
tgataaaggcaatttgaattccctccaataacagaatatacccacaagacgggcaatgccacactggtgactttctgaccagcgacgacattgagaagca
ttgtccagaagtggtaattggcaatgaaaacagcagagaacaatcagcattgactataaaagtaaaaagttcgtccaatctaatccaaagagccacgtag
gaggttcatcgtaagagatatgaatataatggagatatcagcggtggtgaatgaagattattgctgataggacagttccatcagagatgtaagtgcctct
gctggaaaacccattgccgtcattgtcctcaatacaagagcaacatatctacggcagaagtgacagcacaaaaagctttgctctggaaaggaaactgtca
aggggaatcccaatgactcatgttggttcttaatgtaaaagtgaagtggtttagcggctcgtctgtattttataaattgtgtgggacggttgctatgcaa
acagactgttatatcgaatgacacattctaaagtcatttccgaataatctctctgttgtactcactgagctgtggctcagccaacaagattctagccaga
gtttaaagccgctacgtggacttgtgatgtggatgaaaatgattagtgttggttaaaagcatggtggtcaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: