XB-CLONE-4352964
Clone Name: IMAGE:7026365
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Gene:
rfc5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084510
[+]
GAGGAAGCTGCGCGCCAGGACCTTATGGTGCCTAAATGCCTCGGGAATCTGTGGCTGCAAATTAGTTTCTACACGTAGGTTTCGTTGCGGCTTCCTATCC
GCTCGTCATGGTGTCCGAGAATAAGGGACAGCCCGGGCAGAGCAGGAATTTGCCATGGGTTGAGAAATACCGACCACAGACTCTTGATGACCTGATTTCT
CATCAAGACATATTAAGCACAATTCAGAGGTTCATCAGTGAAGACAAACTCCCTCACCTTCTATTCTATGGACCCCCTGGGACTGGGAAGACATCTACAA
TTCTTGCCTGTGCAAAGCAGCTTTACAAAGATCGAGAATTCAATTCTATGGTTTTAGAGTTGAATGCTTCAGATGACCGGGGCATAGATATTGTCAGAGG
ACCAATTCTAAGCTTCGCAAGTACCAGAACCATTTTTAAAAAAGGCTTTAAGCTTGTGATCTTGGATGAAGCAGACGCAATGACTCAGGATGCACAGAAT
GCTCTGAGAAGAGTGATTGAGAAATTTACAGAGAACACTCGCTTCTGCCTTATCTGCAATTACCTCTCAAAGATCATACCAGCTCTGCAGTCCAGGTGCA
CCAGATTCCGCTTTGGACCTCTCAGTCCAGAGATGATGGTTCCTAGACTTGAGCATGTCGTAAAGGAAGAATGTGTAGATATCAGTCCTGATGGAATGAA
GGCCTTGGTGACATTGTCAAATGGAGACATGCGCAGATCACTAAATATCTTACAGAGTACAAACATGGCTTATGGGAAAGTAACAGAGGACACTGTCTAT
ACCTGTACGGGCCATCCTCTGAGGTCTGACATAGCCAATATTCTTGACTGGATGCTGAACAAGGATTTTACTTCCGCCTACAAAAATATTATGGAACTGA
AGACATTGAAAGGCCTGGCTCTTCATGATATTTTAACTGAAGTTCATCTCTATGTGCATCGAGTGGACTTTCCAGCATCGGTTCGAATGCACTTGTTAAT
CAAGATGGCTGATGTAGAGTACAGACTGGCATCTGGGACAAGTGAGAAGATTCAGTTGAGCTCTCTGATTGCAGCTTTTCAGGTGGCCAGAGATATGGTT
GCTGCTGAAGCCTAGAGCCACAAGAAATGAAGAGTGGGCACTGAGCACAGAAGTATTTACAGTAACTTCACAGCAATTCTAAGAACAAGAGATAAACTGA
CAGAACCCACAATTTTATTAGAATACAATCATATAGTGAAGTATGTTGAGGGAAGTGGACATTGTACATATGTGAATTCATTATTTGTTATGAACAATTT
TTTCTAAAATAAAACTAATTCCTGAAGTACAAATGTATCATAGCAGTGCCTGGATCAGCCTTTCAATACAACTTCTGTATTAAAATAGAAATCTCAAGAT
GGTATAGACTGATCTTCCTAGTTTCAAATTAATTAGCTGGTGCCTTAGTGAACATAAAACAAGGATTGGAGTCAGTCGCTCCTCGCTGAATCATGGTGGC
ACAAGCTAGACTGTCCCTCAGGAAGTGAGAAGGGGCCACATCTGCTACTTGAGGTATTGGCTGACAGCACCTGATGGAGATGCTTAAAAGGGAATGCCTG
CCCAAAAATGTTTTTTTCATAATGATAATTTTAAAGAACATTTCAATAACTTTTATTTACTTCCTAATTCTACATTGGTTTATGTAACTGCTATAGTAAG
CCACATTTTCTGTACATAGATTTTTCCCTACAGTGCCTTGCCCACTGTCTTGGCTGGAGGTGAGCTGAGTGCCAATAAAATCCCTCAGTGGAATGTCATA
ACTGCAAACAATAATTGTATATTGAAAAATTGCTTAGCATTTTCTCATTAGACAAGAAATAAAATCATTTTGGGACAGTTAAAAAAAAAAAAAAAA
5' EST: CF783914
[+]taattaatcccccccccccccgaggaagctgcgcgccaggaccttatggtgcctaaatgcctcgggaatctgtggctgcaaattagtttctacacgtagg
tttcgttgcggcttcctatccgctcgtcatggtgtccgagaataagggacagcccgggcagagcaggaatttgccatgggttgagaaataccgaccacag
actcttgatgacctgatttctcatcaagacatattaagcacaattcagaggttcatcagtgaagacaaactccctcaccttctattctatggaccccctg
ggactgggaagacatctacaattcttgcctgtgcaaagcagctttacaaagatcgagaattcaattctatggttttagagttgaatgcttcagatgaccg
gggcatagatattgtcagaggaccaattctaagcttcgcaagtaccagaaccatttttaaaaaaggctttaagcttgtgatcttggatgaagcagacgca
atgactcaggatgcacagaatgctctgagaagagtgattgagaaatttacagagaacactcgcttctgccttatctgcaattacctctcaaagatcatac
cagctctgcagtccaggtgcaccagattccgctttggacctctcagtccagagatgatggtttcctagacttgagcatgtcgtaaaggaagaatgtgtag
atatcagtccctgatggaatgaaagcctttggtgacaatgtcaaatgggagacatgcgcaagatcactaaaatatctttacagagtacaaacatggcctt
attgggaaaaagtaaccgaggaaccctgtc
3' EST: CF783915
[+]tttttttttttttttaactgtcccaaaatgattttatttcttgtctaatgagaaaatgctaagcaatttttcaatatacaattattgtttgcagttatga
cattccactgagggattttattggcactcagctcacctccagccaagacagtgggcaaggcactgtagggaaaaatctatgtacagaaaatgtggcttac
tatagcagttacataaacccatgtagaattaggaagtnaataaaagtattgaaatgttctttaaattatcattatgaaaaaacttttttgggcaagcatt
cccttttaagcatctcaatccagggctgtcaggcaaatacctcaagtagccgagtggcccccttcccactttcctgaaggaaaatccaaactttgtcccc
ccctggttctcgcgaggaaagcgcctgaacccccatcccttgtttttggtttcccctaaggggcccccccttttttttttttgaaaccctgggaaaaaac
cgtccctaacccccctttggaaattttctttttttaaaaaaaaaaattttttttttttaaaaggggtaatttcccgcccccccccgcctttaaaaaaatt
tttttcccccccccggaaatattttttttttttttttaaaaaaaaattttttttctacaaacaaaaaaaaaaagaaatctcccccaagtggggaagaggg
gccccccttctcctccccccaaaaaatttcccccaaaaagaaagaggggggtgtccccaaaaaaaaaaatggggggggttcctccccaatatttatctcc
ctcgtttttttaaaaaaagctgggggggagaaacctcgaaaaaaaattttgtggggggggggggggcccccccccatttttttggggggggagaggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: