XB-CLONE-4352961
Clone Name: IMAGE:7026364
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Gene:
slc3a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783917
[+]
taattaatcccccccccccggtcacacaaagcccttactgacagctgcctggagcgtgagcacaagacaccaggacaccgacatgactcaagataccgcc
ctagacatgaaagacgttgaactgaatgaattggatcaagagaaggtcccaatggcaggtggggctggggacagcccaactggagggggagagaagaatg
gcgttgttaaggtgaagcttgaagatgatgatgcgattggaaccaagagtcagaaatttacagggttgtctaaagaagaattactgcgagtagctggtac
cccagcatgggtgagagtccgatgggcattgctgatcctgttttggttaggctgggctggcatgctagcaggagctgtggtcatcattgtacaagctcct
cgctgccagcctttgccagccatggaatggtggaacaagggacccctttaccaagttggagatcccgcaacctttcaagaagatggggctggaaatatcc
agagtatagagaagcgccttgaaccacttaccagtctgaaaataaatgggtttgattattgttccaatacatgcttacaaagaaagatgaaattgaccaa
accttaccccctggataccgaaccctaatcatgggcctcatggacccaatttccccagtcctgccggtaagctttcccgcaaaaaaaaagtattcccgga
taacatctttgggaacctcatttcccttaatttcccgccacctggaaaaaaaacctctgtgtttttttaaaccgaactggacaccttaacctccttgtat
gggatataaccgtcaatccgcgttaatattaccccccacgaaaactagtgggcctcgggaaacacccccgcccggttttacgcaaaaggtttacccccat
ccatttttttgggtaa
3' EST: CF783916
[+]tttttttttttttttctttttttttttttttatgaaacaaacactaactttattgaagtaggaattttttgctgaccagcaccatgttgcataactgtaa
ccaaactttcccctccccaaatacacaagatccacagacattcatattcccctggctgcggcccatctctccatgctcagagggctaaaagtgccacagc
ctttaaaacatgaaatgacaacataaaaacacacctcagagcacgtgtatgacaaaactatgcaaaaaacaacactccacttattgctcttaacgcgttt
cagaaactgtttttaagtttttatcctctgaacatgaaaaatatgaccgcttggcaaatataaatggcaggttacagtatatgccactgccacaaagggt
agggatgtgttaaaaggtttaaagggaaggttaacaacatgtgcttcttggcactggaaagaagggtcagcaagtggaatgtgtaagtgtttgcccctca
cagggatgtagggggttatcccctataagggtatttaaagagtaaaagcctctccagctcctaaatgggaagcttttttagggaaaaacttttccccttc
tttttccttgggagcctggaaactcaacaactactttggccgtgtttttgggaaaacccttccgccctcgggtccttttttcaagggggaagagttctac
ccccgtcccccccaaaatttaaagtttccaagggcgggcaacataagggctccaattacttggggcccagactccctttaagaaagagaaatagggccct
gtgtcaccgggttaaaacagataaaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: