XB-CLONE-4352955
Clone Name: IMAGE:7026362
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Gene:
eif3b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783921 [+]
taattaatcccccccccccccgccccttttccgccggtgcccggtacagcgcacatggcctgttagtagcgccaagccttgcgttctctttattctcggg
gagaaacgaattccaaccacggagagacgcaagaatccaacgtaggtagaacagagacaaacaggaaagtgccgaggaatgctggagagcgaacgacccg
agagagacatggaggaggaagagtccaccgaggaggaggaggaagaagggatgtcctttagcgatccggagggattcgaggacgatatcagtgaaaagga
gttgcttggtgatatcctgaaagaccgtcctcaagaggcagatggaattgactctgtagttgtggtagataatgtgccacaagttggacctgaccgcttg
gaaaaactcaaaaatgtcatcaataaaattttctccaaatttggtaaaaattccaatgaataatatccgggaagcacaaaagcaccaccgaggggaaaca
ttttttctggaaattggctttgcccaactccagggcgcaaaaaagcaagaaaaaagaaacggaaaaattggttaccaaaaatgggaaacaaagcaaacaa
caacaatttcccggcgattgaaaatcttccttaccctcggaactctttcgtacaaaaagtacaaattgggtctctttatttggggagaagataaaaaata
tggggggaaaaaggacaacccctggggaaagaaaaaacatataacccctttttttttaaaataagaaatattttttttgggggggaaaaaatatattgtt
agagggcgtattccatatttgggggagcatnatttttgaaagggggaattgtacacctcttggtaaaacatttcgagctcccctcctaaaacatatattc
gcgcgtgtttttctgcacggccaggctttatctataactntaaatatgcgggataaaagattccgtggtgtggtgggtgaaaaattcactctgacacatc
tcactcataataataacgcataacccaggggggggaacatgttggagcatatttttattataaaagaggggtagtttccccccgcggtttactcccccca
actttttttacataagggcgcagaaagcaaaaaaaactacataaaataatttggtgaacactagtgggcaagaattatatttattttgtgtattcccctt
ccgcgtggctgcacctcccacaatatgaaaaagacgaaacaccttttgacccccctctgtgtggcaaaaaacatattatgcgagatttgacagatggggg
tgagagagaaccatactcgcctcctcccgttagtgtgagaaatgggagtgaaggggaaaaacagatttatttaagcacaccataaaatattgttggtgga
gnggaatttttnaaatcccactaacactgaagggggtgg
3' EST: CF783920 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttaaaaaaaagggttaa
tttaaaatcccccctaaccccccatttggaaaacccggagggggggggaaaaaacccccgggaagggggggttaagggcccaaaaggccggggtggcccc
ccctttttggttttcccccggaaaaaaaaaaggggggagggcccttttgaggggtaaaaaaaaacccccctcccaaaaaaaacccccggggccaaaaaag
gggaaaaaaaattttggggggggggaaacgggcccccaaaaaaaccccccccccaaaaaaccaaatttttttaaccccaaaaggggccaagggggcgggc
ccaaaaaaaaacccccccctttcccaattaaaaaaaaggccctttaaaaaaaccccccagggggggcaaagggcccatctccaaaaaatttattgtcccc
ttccgggggggcagaaaaaaaaaaaaagggaacttggggagtcccccccccggacaaaaaaaactcccaaaaaatgtggggaaaaatctttaacaaaaac
ccctccccgggggaaaaatttttttcatataaaaagaaaacccaaaggcccttctccccccaaaaaaaacaaaaaatgggggggtcccccccaccttggg
tggttccccccctcctcctttgatatcccaaaaaggggggggctcttttggtttcccagaggaaaaagatattgggggcccccactccccccggagggag
gtcccgggataaatctaacacacacaggggagcgccatgtcgcgcgccaccaaataaaaacacccccccgcggtgagc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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