XB-CLONE-4352952
Clone Name: IMAGE:7026361
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Gene:
adissp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783923
[+]
taattaatcccccccccccccaagacgcagtgctcaagttcagtcacagcccgggactggagacgggagcttgtctggcgggtatcgctacgcactcggg
agcgcacagtgctttgacaggacctagaactaaaggaggatgagagccttttgatccaatcggaccctatacttgtgcaatcaatggcggctgctaaaaa
gggtgcaaagccgaaagtggccggggttcgcttcgctgcaggatttcctgaagaaggggcgcacaatcacgtccattttgatgaacagctgcacgactcg
gtggttatggtgtcccagcaggaagatgggaattttttggtcaaggtgggtttcctgaagatcttgcataaatacgagatctccttcagcttgccgccag
tgcagagactggggaagagtatatgcgcggtccctctcccgaccctcaacctgaaagtgctaagcataacctcgcaggcagaaggacacagcattaagtg
tgagtacacagcgcataaagagggcgtgctgaaagaagaaatgatacttgcaagcgaaaccaacgacaaagcctttgtcaaggtggtggttcaagcaaga
gttctggatcgacaccccgggacccccaatgctgctgggaaggcgtccgatgtaccggaacttgagctgggagtacaactcccaaacaagaacgacttgg
catgggaattcgactaggagggcggtcaagcttacgccaaacaaggcccgaaaggttttcccccttgtcaacctcttggcccgtcctaaacaaagaaaaa
aagccctgtgaaaaact
3' EST: CF783922
[+]tttttttttttttttatttttttttctattcactattttttttctttcccacttctgaaccatctgtattacaggtacctaaaacaagccccgcccctct
attcccgccattcttcaggcagtccattgtaccactgtccattgtaccatacagagtaataaagcataggggtaaatagatactcagcagcgataggcgc
agccagtcctacacacattgcagcacacaagcaagttacacgcagagagagcggaaccaatggccgccgccggatctataataacgaaacagacaggaac
acgggacattagttacattagtgcaagctgacaatgagtaggcccaagtccagcacaaacggtactgatccccctcgtctccatcagccaccaatcgtgg
tgccccattgtagggcccctacatttctccgaagggggcgggacaatgacccgctaatcaacgtcagagattcccatcctgttttatggctgtcaggctt
ttcttgttggacgcagagttgcatgggaaaccttcggcctgttcgctagcttggcgcctctagtcgaatccatgccagtcgctctgctcggagtcgtact
ccagctcagttccggtacatcggacgccttccagcagcatcggggtcccgtggtgtcgatccagaactcttgcttgaaccaccaccttgacaaaggcttt
gtcgttggtttcgcttgcaagtatcatttcttccttcagcacgccctctttatgcgctgtgtactcacactntaatgctgtgtcccttctgcctgcgaag
tt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: