XB-CLONE-4352899
Clone Name: IMAGE:7026342
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Gene:
septin10
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084509 [+]
CGGCTTGTGACTTTTCACTGGAAAAGTTATGGCAGCTTCGCCTGAGCTCTCCCGGCATATGGTTTACCAATCAAATCTTGGAATGAGATCTCCCCAGAAC
AGCAATCCAGACATTCAAGATGCTCACAGACATAATGAAACTTCTAGGAGCTTGCCTTTATCTGGACACGTTGGATTTGACAGTTTACCTGACCAATTAG
TTGAGAAATCAATTCGCCAGGGATTCTGTTTCAACATACTTTGCATTGGGGAAACTGGCATTGGAAAATCAACATTAATTAACACACTATTCAACACTAA
CTTTGAAGAACAGGAAACCAGCCATTTTGAGTCATCCGTAAAGCTAAAAGCACAAACCTATGACCTCAAGGAAAGCAAAGTTCATTTAAAATTGACTATT
GTAAATACAGTTGGATTTGGAGATCAAATAAACAAAGAAGACAGCTACATGCCTATTGTGGACTACATTGACAAACAGTTTGAAGTGTATCTGCAAGAAG
AGTTAAAGATTAAACGTTTGCTATATGCATATCATGATACTCGTATTCATGCCTGCCTTTACTTCATTGCACCTACTGGCCATTCCCTAAAGTCCCTTGA
TTTAATAACTATGAAAAAGCTTGATAGCAAGGTAAACATAATTCCTGTCATTGCTAAAGCCGATGTGATTTCTAAATCAGAACTGCACAAATTCAAAAGT
AAATTGATGAGTGAACTTATCAGCAATGGTGTTCAGATATATCAGTTTCCTACAGATGATGAAACTATTAACAAAATTAACTCCTCTATGAATGCACAGC
TTCCATTCGCAGTGGTAGGAAGTATGGAGGAAGTCAAGATAGGAAACAAAATGGTGAAAGCTCGGCAATATCCTTGGGGAATTGTACAAGTGGAAAATGA
AAATCATTGTGATTTTGTAAAGTTACGGGAGATGCTGATTAGTACTAACATGGAGGATCTCAGAGAACAGACCCATTCCCGGCATTATGAGCTGTACAGG
CGCTGCAAATTGGAAGAAATGGGATTCCAAGATATGGGACCTGGAGATAAGCCAGTAAGTTTGCAGGAGACCTATGAAGCTAAAAGACAAGAGTTCTTTG
ATGACCTTAAGAGAAAGGAGGAGGAGATGAGACAGACATTTGTGCAGCGAGTAAGAGAGAAAGAGACTGTGCTGAAAGAGGCAGAAAGAGAGCTGCAGAA
CAGATTTGAACAATTGAAAAGGCTCCATCAGGAGGAAAGAGAAAGCCTAGATGAAAAGAGGAAAGAACTTCAAGAAGAGATGAACAATTTTAGCAAGAGA
AAGGCAGCTGCAGAATTGCTGCAAGCCCAGGCCTTCGGAAATGTTGCCAATACTAGTTTGAAAAAAGATCGTAAAACATTCATGTAAATGGAAGATGGTA
AGATGCTGCATAGATTAAACAGCTACAGGCCTGCCATGAGTGTCATTTGTATTCCATTTCCCTGTATTTTTTTCTGTAAAATTAATTGTATTTGATGTAT
CGATGCTCAAGGAGGATATTTTGGGATAACACTCTGCCAAATTTAAAGCGTTTAGCAGCACCACAATCAACACATGTCTCACTGTAAGCGCTTCGGTGTC
TAATTACCTTTTGTAAGGCTGGACCTTTTGAGCCTTTCCACTACTACTGCATGGAACCAAAGAGAAATGCAGAGAACTGCTTTCAGCTGCAGGAACATGG
GAAGATTTTCTACCATTATAAACAAAAGGGAAAGGACATTTCAGTGTATATGTTTACTTTTTTATATTTTCCTATTATGAGGTGTATTGCAATAAGTTAA
CCCTTTCACTGGTGATAGCACAGTTACCTGCCATTACCATGCATCAGATATCACTCCATTTGTATCTAGTTGAAGTAAATCTAGAGCAACTGGTAAAAAA
CGTTTTACTACTGACAGGAGCAGTTTCTTCATTTCCGAACTGAAGAAGCAGCTCAATTAAGTAGTGAAGTGTCTTCAGTGATTACTGAAGTCCGGTTGCT
CTAGATTTACTTCTACTAGAAATACCATGACCTGGATGAATGAAAACCTTCATAGACATCACTCCATTTGCTTTTGCAAGAAGAAGGAATAAAGAAGTGT
GCGTTGAGACCAAGGAGGTTTTTGGATTAGTAGTTCTGCAACTTCTGCTGATGCTATTCCATCCCTATTTTAGATACATTTTTCTGCACTGATCTAATGT
GCACTAAAAAAATCCAGCCTTACTCACCATGTAGAAAAAGTAACCCTTTATTGTGGACAAAGTATATTGATTTCCTTGGCCCCAATGCCTTATTATTAAC
TTTGCTGACCGCAGCATAATAATGAAAACAGGTAACCGTAAATGTGCATTATTTCAATTTAATGATACTTCGAATTACTTTGTTTAGCACCCCTGTGAAA
TGGTTTTCTATTTTCTACAAAAGTGGAAAAGAATGCTTTTTCTATGTATATTTTTTAACATTAACCCTTTCTATGAATATTATAGGTATTATTCTAAAAA
AAATATACTTATAAAAAATAAAGATTTTAAACACCAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF783957 [+]taattaatccccccccccccccggcttgtgacttttcactggaaaagttatggcagcttcgcctgagctctcccggcatatggtttaccaatcaaatctt
ggaatgagatctccccagaacagcaatccagacattcaagatgctcacagacataatgaaacttctaggagcttgcctttatctggacacgttggatttg
acagtttacctgaccaattagttgagaaatcaattcgccagggattctgtttcaacatactttgcattggggaaactggcattggaaaatcaacattaat
taacacactattcaacactaactttgaagaacaggaaaccagccattttgagtcatccgtaaagctaaaagcacaaacctatgacctcaaggaaagcaaa
gttcatttaaaattgactattgtaaatacagttggatttggagatcaaataaacaaagaagacagctacatgcctattgtggactacattgacaaacagt
ttgaagtgtatctgcaagaagagttaaagattaaacgtttgctatatgcatatcatgatactcgtattcatgcctgcctttacttcattgcacctactgg
ccattccctaaagtcccttgatttaataactatgaaaaagcttgatagcaaggtaaacataattcctgtcattgctaaagccgatgtgatttctaaatca
gaactgcccaaattcaaagtaaaattgatgaagtgaactttatcagcaatggttgttcaaaatatatcaagtttcctaccgatgaatgaaacctatttaa
caaaaatttaacttcctccaatgaaatgcaccagcctttccatttccccaaatggtgtaggggaaattaatgggagggaaaaatcaaagaaaaggggaaa
aaccaaaaattgggtggaaaaa
3' EST: CF783956 [+]tttttttttttttttctttttttttttttttttggtgtttaaaatctttattttttataagtatattttttttagaataatacctataatattcatagaa
agggttaatgttaaaaaatatacatagaaaaagcattcttttccacttttgtagaaaatagaaaaccatttcacaggggtgctaaacaaagtaattcgaa
gtatcattaaattgaaataatgcacatttacggttacctgttttcattattatgctgcggtcagcaaagttaataataaggcattggggccaaggaaatc
aatatactttgtccacaataaagggttactttttctacatggtgagtaaggctggatttttttagtgcacattaaatcagtgcagaaaaatgtatctaaa
atagggatggaatagcatcagcagaagttgcagaactactaatccaaaaacctccttggtctcaacgcacacttctttattccttcttcttgcaaaagca
aatggagtgatgtctatgaaggttttcattcatccaggtcatggtatttctagtagaagtaaatctagagcaaccggacttcagtaatcactgaagacac
ttcactacttaattgagctgcttcttcagttcggaaatgaagaaactgctcctgtcagtagtaaaacgttttttaccagttgctctagatttacttcaac
tagatacaaatggagtgaaacctgaagcatggtaatggcaggtaactgtggctatccccaagtgaaaggggttaacttaattgcaataa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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