XB-CLONE-4352896
Clone Name: IMAGE:7026341
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Gene:
pdhb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783959 [+]
taattaatccccccccccccgacgttttgtccagaggacagaagtaagatggccgccctcaggagcttgttctgcggagggaagagctcggtgtccgtgt
ttctgaagagaagcttccacagaagcaccccggccgccctgcaggtgactgtacgggatgcattgaaccaggctatagatgaagaaatcgagagggatga
aagggtgttcctgctcggggaggaggtggcccagtatgatggagcctataagatcagtcgggggctctggaaaaagtatggggacaagagagttatggat
actcccatatctgagatgggctttgctggaatcgctgtaggtgctgccatggctggcctaaggcccatctgtgagtttatgacctttaacttctccatgc
aagccatcgaccaggtgatcaactctgctgccaagacctactatatgtccgctgggcttgttccagtccccatagtcttccggggacccaacggcgcgtc
ggccggggtggcagctcaacactcgcaagtgctttgccgcctggaatgcacactgccctgggcttaggggggggagcccctgggacgcagaagatgctta
aggtctccttaagtctgcttttccggaataaaacctcagtggtgttcctggaaaaagaattcgatgtaccggggcccccctttcgattcatcccaagccg
ggctcccccctaaagaaccactttccttttctggaatgggggaatacccctaagacttggcggcgggccaggggcccctcacaattaatatctgagattt
tcctttattattcccgccttccggctgtggggcctatgtgt
3' EST: CF783958 [+]cctacaggagattcagagtcttctttactgcaaatataatatccttcacttgagggacgcagttctcttccaggatcttggcgtaaggcatcgggacatc
agtgccagtcacacggaccactggcgcatcaaggtaattgaatgctggcccttccatgatcttggcacagatttcagagcctacgccaaactgtggccaa
ccgccttctactgtcaccagatggtttgttttcacaacgctcgtctcgatggtttcaatgtccatggggcgaatcgtacgcatgtttattacctcacagt
ctactccctccttggccaacacgttggcagcctccagacagtggccaacagagcgggagtgagaaaccagtgttatctgggatcctggtcgctctatctt
tgctttcccaatcggaatgacatagtctttagactgagcctgctcggataactcaaaagggaccccgtacatcaactcattttccaggaacaccactggg
ttattatcccgaatggcagacttaaggagacctttagcatcctctgcgttccaggggctcaccaccttaagcccagggcagtgtgcataccaagcgggaa
atcactgcgagtgctgagctgccaccccggccgaaacgccgtttggggtcccctgaaaaatatgggggactggaacaaacccagcggacattatattagg
tcttggcaacccaaattgatccccctggtctcaaggccttgcatggaaaaaatttaaaggttattaaacctcccaagattgggcccttaaggcccacccc
atgggcaaccaccttataaccgcatttcctttcccagcccccaaatccccaaaaacacgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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