XB-CLONE-4352885
Clone Name: IMAGE:7026337
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Gene:
slc35a1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084147 [+]
CAGTAGTAGCTCCAGGAGCCGCTTTAGAGAAGTGGCAGGATGGCAGCTAAAGAAAATGTCAGTCTGCCCTTCAAGCTCTATTGCTTGCTGGTAATGACCC
TTATTGCTGCTGCATATACTGTAGTTCTTCGCTACACCAGGACGGCAACAAAAGAAATGTACTTTTCCACAACAGCCGTATGCATCACAGAAGTTATTAA
GTTGCTCCTGAGTGTGTGCATTCTGGCAAAAAACTGGAAGTCTTAGTAGATTGATAACTTCTTTAAAGGACAATGTATTGGGGAGTCCTGTGGAGATGCT
GAAGCTGTCCGTACCATCCCTGGTTTATGCTTTGCAGAATAACATGGCTTTTGTAGCTCTGAGCAATCTTGATGCTGCAGTGTATCAGGTCACTTACCAG
CTGAAAATCCCCTGCACTGCATTGTGTACTGTATTGATGTTGAACCGCTCACTAAATAAGCTGCAGTGGGTTTCTGTGTTCATCTTGTGTGGAGGTGTCA
CCCTAGTGCAGTACAGTCCAGCAGAAGCCACTAAAGTACAGATTGAACAGAATTATTTACTGGGCATAGGTGCTGTTGCCATTGCTGTGCTGTGTTCTGG
ATTTGCAGGGGTTTATTTTGAGAAAGTTTTAAAAAGCTCAGACACTTCCCTGTGGGTGAGGAACATCCAGATGTATCTTTCTGGCATTTTAGTGACGGCA
TTATGTGTATACATATCTGATGGATCGCAAGTCATTGAAAAGGGCTTTTTCTATGGCTACAATTTCCTAGTATGGATTGTCATTTTGCTTGCAAGTTTTG
GAGGCCTCTACACATCAGTTGTTGTTAAATACACAGATAATATCATGAAGGGATTCTCTGCAGCAGCCGCCATTGTCCTGTCCACCATTGCATCAGTGAT
TCTTTTTGGTTTACAGATAACTTTAACGTTCGCCATTGGTGCACTGTTTGTTTGTGTGTCAATATACACATACGGATTGCCAAGAAAAGACACAACTACG
ATAGACGTAAAGGAGCTTAGCAACAACCTCAGTGGAAAACTGATCAGTGTGTGACGTTTGCCAGAGCATGACCGCTGAGACTGTTACAGAGGTAACCCAG
AAAAATCAAGGACAAGTTGCATTACTCCTTGCTTCAGGCCGTGAAGAAGCAGGCAGTATTTGGGCCTGTCCTGGCATGGCTTTAAAAACCATGCTTAAAG
CTGAATGTAACATGTGCAGGACTATATCATATGGAAGGCCAAATCATGTATGTCCCAAGCAGTGGTGTATTTAATTGCTCAGTATTGCTTTAAGTTAACT
GAAAAGTTAACGTCTTGTAAAAGTTACAATGAAGCAAGTAGCGTATGAAGAATTTTTTGCAAAGAGAATGCCAAATGAGGGTAAACGACTGCAACTCATT
AAAGAATTAGTAGAACTAATTGTGTACTGCATGCAAGTAAATGGCCAGTGCCAGTGGAATATGCTGCAGTTGTGCCCAAATGAAGAGACTTACGCTCAAA
TGCAGACAGATTTTGCCATCTTTAGAATATTCTTTTTGTTTTCACTGCTGCAGTATTTTCAGACTTGGAAACATGTAGTTATTTTATTTGCACTTTTTAA
ATTTTTTATGGGATTGTTAGACTGAAGGCCAAGATTTTTTTTTTTTTTTTTTTAAATTATGAAGGTAATAAAATTTTCTCAGTAAAATTTGAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF783966 [+]taattaatccccccccccccccagtagtagctccaggagccgctttagagaagtggcaggatggcagctaaagaaaatgtcagtctgcccttcaagctct
attgcttgctggtaatgacccttattgctgctgcatatactgtagttcttcgctacaccaggacggcaacaaaagaaatgtacttttccacaacagccgt
atgcatcacagaagttattaagttgctcctgagtgtgtgcattctggcaaaaaactggaagtcttagtagattgataacttctttaaaggacaatgtatt
ggggagtcctgtggagatgctgaagctgtccgtaccatccctggtttatgctttgcagaataacatggcttttgtagctctgagcaatcttgatgctgca
gtgtatcaggtcacttaccagctgaaaatcccctgcactgcattgtgtactgtattgatgttgaaccgctcactaaataagctgcagtgggtttctgtgt
tcatcttgtgtggaggtgtcaccctagtgcagtacagtccagcagaagccactaaagtacagattgaacagaattatttactgggcataggtgctgttgc
cattgctgtgctgtgttctggatttgcaggggtttattttgagaaagttttaaaaagctcagacacttccctgtgggtgaggaacatccagatgtatctt
tctggcattttagtgacggcattatgtgtatacatatctgatggatcgcaagtcattgaaaagggctttttctatggctacaatttcctagtatggattg
tcattttgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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