XB-CLONE-4352859
Clone Name: IMAGE:7026326
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Gene:
p4hb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783982 [+]
taattaatcccccccccccccccgctgccttcagaacgccacgtcctgtacacccggctcatcatgatgcgcgcagttcttctattcggctgttccctat
taatcgtcgccagggccaatattcccgaggagcgggatgtactggtgctaaagaaagacaactttgatgaggcgttaaagcagtacccgtttattctagt
ggaattctatgctccctggtgtggtcactgcaaggcactggctccagaatatgaaaaggctgctggcgtattaaaaagtgaaggtttgccgatccgcctg
ggcaaggtagatgctacagaggagtctgatctggctcaggagtttggcgttagagggtacccaacaattaaattcttcaaaaatggtgacaaggcttctc
ctaaagaatattcagctggcagggaggcagcagacattgtcaactggctgaagaagcgtactggcccagctgccagcactttaggtgatgaggcaggtgt
tgcagctttggtggattccagcgaagtcgcagttattggtttctttaaggatccagcgtcagagccagctaaagtgttcctacaagcagcagaggcagtg
gatgatattccatttggtatcacatccagcgaggcagccttttcaaaatatgaacttggcaaggatggcattgttctgttttagaagtttgatgaaagcc
gaaatgcatatgagggagatatcacccaaggaagaagttttgagccttcattaaaggccaaatccgtttgccctctgggttaatagaattttactgaagc
aaaactggctcccaatggatcctttggg
3' EST: CF783981 [+]tttttttttttttttgttaataaaattaggtttatttggccaacactttgttccaacttaaatatgatggaatgcttcaagaatcaaacagattctactg
gaaacatcctcctgtccagagaaaatggttaggtttgcttttggaggatgggtcaagccccagacaggacagaagtatcatttataaccgagctatacca
ccaaggtttggttgggagcagcgaacactggaattacattgttggtagtaataatggaacaggggattctaccagtattccgtcaatgttttaaatactg
aaccacaatatctgggtgaccatttgtcctggagggagacactttcaaaaagggagaaaaaaaacaaaaccaaacaagacacccacatcccatacccata
gttacggcagcaaacagcagcttaggaggttaagctctcaccagtcatgcattccatcccatccacaataaagctcacaattaaaaaaaacaaaaacaaa
taaaaacagtcaaaaaagttacataaagcatcaagtgctagacatcagaataattcaaattaggggtttaaacaaaatagcatcagtggaaaacaaacaa
aaaaaaaaaaaaaatgacgaccattaacacaaggtcaaaagttcccttttccctctcagacaacagctgaagtgagatttccctgcaatttcagagcttt
gacacagtaacatttctagacgtccacccctcttagntgtcttctggtttctgcaagttagtcttattcttataattcaatcttttggatgtgcttcatc
tccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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