XB-CLONE-4352848
Clone Name: IMAGE:7027560
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Gene:
psmb3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783989
[+]
taattaatcccccccccccccccgctcagcgttactggaaccggctgctggagctttcggttttgtatccggcggaggcacagaactatcgctaacatgt
ctattatgtcctataacggtggggccatcatggccatgagagggaaggattgcgtggccatcgctgcagagcggcgcttcggggtacaggcacagatggt
aaccacagactttcagaagatcttccctatgggggaccggctgtacatcggattggctggcctggctacagatgtgcagactgtgtcccagcgcttgaag
ttccgactcaacctctatgagctgaaggaaggacgtcagattaagcccaagacgttcatgagcatggtggctaatctgttatatgagagaaggtttggcc
cttactacatagaacctgtcattgctggcttggaccccaaaaccttcaagcccttcatttgttccctggatctgattggatgccccatggagacggaaga
ctttgtggtgagcggaacctgctctgagcagatgtacggaatgtgcgagtccctgtgggaaccagacatggagcctgaggacttatttgagacaatctca
caagccatgctcaatgctgtggacagagatgcagtgtcttgggaggggagtcgtcctacttgtgatagaaaaagattaaatttcccccccggactctgga
gggcacgcatgggattaactcccctgccttgcccccgggattctggcctctgaataaaaggtggtttggctttccacccctttgcctttttgattaaaaa
c
3' EST: CF783988
[+]ttttttttttttttcttttttttttttttgaagatatttagttgttttttttaattaaaaaaaagaaaaaaaaaaagcaaacaacattaatcagaggcag
aatccggggcaagcagagagttaatccatacgtgccttcagtgtccgcgtggttattttatctttttctatcacatgtacgacgactcccatcccagaca
ctgcatctctgtccacagcattgagcatggcttgtgagattgtctcaaataagtcctcaggctccatgtctggttcccacagggactcgcacattccgta
catctgctcagagcaggttccgctcaccacaaagtcttccgtctccatggggcatccaatcagatccagggaacaaatgaagggcttgaaggttttgggg
tccaagccagcaatgacaggttctatgtagtaagggccaaaccttctctcatataacagattagccaccatgctcatgaacgtcttgggcttaatctgac
gtccttccttcagctcatagaggttgagtcggaacttcaagcgctgggacacagtctgcacatctgtagccaggccagccaatccgatgtacagccggtc
ccccatagggaagatcttctgaaagtctgtggttaccatctgtgcctgtaccccgaagcgccgctctgcagcgatggccacgcaatccttccctctcatg
gccatgatggcccccccgttataggacctaatagacatgtttagccgatagttctgtg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: